IEC
46
TOSOH BIOSCIENCE
TSKgel Cation Exchange Columns
TSKgel
Matrix* Particle Pore
Functional
Counter
Excl. limit,
Capacity Small ion pKa Column
size (µm) size (nm)
group
ion
PEG
**
(Da)
(mg/mL)
capacity
hard-
meq/mL
ware
***
BioAssist S
pMA
7, 13
~130
Sulfopropyl
Na
+
~4,000,000
70
(1)
0.1
2.4 PEEK
SP-5PW pMA 10, 13, 20 100
Sulfopropyl
Na
+
1,000,000
40
(2)
> 0.1
2.3 S, G
CM-5PW pMA 10, 13
100
Carboxymethyl
Na
+
1,000,000
45
(2)
> 0.1
4.2 S, G
SP-STAT
pMA
7, 10
~ 0
Sulfopropyl
Na
+
500
10
(3)
> 0.023
4.0
S
CM-STAT
pMA
7, 10
~ 0
Carboxymethyl
Na
+
500
15
(3)
> 0.1
4.9
S
SP-NPR
pMA
2.5
~ 0
Sulfopropyl
Na
+
500
5
(2)
> 0.1
2.3
S
SP-2SW Silica
5
12.5
Sulfopropyl
Na
+
10,000
ND
0.3
2.2
S
CM-2SW Silica
5
12.5
Carboxymethyl
Na
+
10,000
110
(2)
> 0.3
4.2
S
CM-3SW Silica
10
25
Carboxymethyl
Na
+
30,000
ND > 0.3
4.2
S
SCX
PS-DVB
5
6
Sulfonic acid
Na
+
, H
+
ND > 1.5
S
*
pMA = poly methacrylate; PS-DVB = polystyrene-divinylbenzene ** Polyethylene glycol
*** PEEK = polyethyletherketone, S = stainless steel, G = glass
(1)
γ
-globulin; (2) hemoglobin; (3) lysozyme
TSKgel Anion Exchange Columns
TSKgel
Matrix* Particle Pore
Functional
Counter
Excl. limit,
Capacity Small ion pKa Column
size (µm) size (nm)
group
ion
PEG
**
(Da) (mg BSA/mL) capacity
hard-
meq/mL
ware
***
BioAssist Q pMA 10, 13
~400
Polyamine
Cl -
>5,000,000
70
0.1
9.4 PEEK
SuperQ-5PW pMA 10,13
100
Trimethyl-amino
Cl -
1,000,000
100
> 0.13
12.2 S, G
DEAE-5PW pMA 10,13, 20
100
DEAE
Cl -
1,000,000
30
0.1
11.5 S, G
Q-STAT
pMA
7,10
~ 0
Trimethyl-amino
Cl -
500
20
0.27
10.5
S
DNA-STAT
pMA
5
~ 0
Trimethyl-amino
Cl -
500
35
0.27
10.5
S
DEAE-NPR
pMA
2.5
~ 0
DEAE
Cl -
500
5
> 0.1
11.2
S
DNA-NPR
pMA
2.5
~ 0
Proprietary
ClO
4
-
500
5
> 0.1
11.2
S
DEAE-2SW Silica
5
12.5
DEAE
H
2
PO
4
-
10,000
ND > 0.3
11.2
S
DEAE-3SW Silica
10
25.0
DEAE
Cl -
30,000
ND > 0.3
11.2
S
Sugar AXI
PS-DVB
8
6
Trimethyl-amino HBO
3
-
ND > 1.2
12.5
S
Sugar AXG PS-DVB 10
6
Trimethyl-amino HBO
3
-
ND > 1.2
12.5
S
SAX
PS-DVB
5
6
Trimethyl-amino
Cl -
ND > 1.0
12.5
S
Properties of TSKgel Ion exchange columns
