IEC
56
TOSOH BIOSCIENCE
Specialty columns
Ion exclusion chromatography can be used as an effective method
for separating alcohols. An example of a saccharide, organic acid,
and alcohol separation is shown in
Figure 17
on two TSKgel SCX (H
+
)
columns in series.
Column: TSKgel SCX (H
+
), two 7.8 mm ID x 30 cm L (in series);
Sample: 1. maltose, 2. glucose, 3. fructose, 4. lactic acid, 5. acetic acid,
6. methanol, 7. ethanol, 8. butyric acid; Elution: 0.05 mol/L HClO
4
; Flow rate:
0.8 mL/min; Detection: UV @ 210 nm, Refractive Index
Separation of mixture of saccharides, organic acids and alcohols
3
4
5
8
UV
45
1
6
7
23
4 5
8
RI
0
30
Minutes
Column:
Sample:
Elution:
Flow Rate:
Detection:
TSKgel SCX (H + ), two 7.8mm ID x 30cm (in series)
1. maltose, 2. glucose, 3. fructose, 4. lactic acid, 5. acetic acid,
6. methanol, 7. ethanol, 8. butyric acid
0.05mol/L HClO
4
0.8mL/min
UV @ 210nm, Refractive Index
15
figure 17
Separation of mixture of saccharides, organic acids and alcohols
APPLICATIONS - TSKgel CATION EXCHANGE COLUMNS
