hydrophilic interaction
CHROMATOGRAPHY
59
hiLIc
hydrophilic interaction
chromatography
hilIC PRODUCTS
silica based hilic columns
TSKgel Amide-80
TSKgel NH
2
-100
The first columns used in chromatography were
glass, both for liquid-solid chromatography by
Tswett in his separation of plant pigments and by
James and Martin in their first gas chromatograph.
However, as the technique developed and particle
size was reduced, the length of the columns in liquid
chromatography was decreased. This resulted in the
columns having to be operated at higher pressures.
To accommodate these higher pressures, stainless
steel columns were introduced. Tosoh introduced
its first HPLC (GPC) columns in 1971, which were
composed of stainless steel. Recently, columns
packed in PEEK, a biocompatable fluorocarbon
polymer, became available. PEEK can withstand the
pressures commonly encountered in HPLC.
TOSOH FACT
