Iec
IEC
49
Oligonucleotides
Figure 2
compares the high resolution separation of nucleotides on
a 10 cm length column to the high throughput separation on a 3.5 cm
length column.
Polymer-based Anion Exchange Columns
TSKgel BioAssist Q is suitable for use in systems that are designed for
laboratory or semi-preparative applications.
Figure 3
demonstrates the
performance enhancement of TSKgel BioAssist Q over a competitive
product when operated side-by-side on an FPLC system.
Column: TSKgel BioAssist Q, 4.6 mm ID x 5 cm L (PEEK),
Competitor Q, 5.0 mm ID x 5 cm L; Elution: 30 min linear gradient from 0
to 1 mol/L NaCl in 20 mmol/L sodium phosphate pH 8.0; Flow rate: 1.0 mL/
min; Detection: UV @ 280 nm; Sample: 1) conalbumin, i) ovalbumin impurity,
2) ovalbumin, 3) trypsin inhibitor
Performance enhancement on FPLC system
BioAssist Q
Competitor Q
10
Minutes
5
15
mAU
mAU
1200
1180
1600
1140
1120
1100
1080
1060
1040
1200
1180
1600
1140
1120
1100
1080
1060
1040
0
Column:
TSKgel BioAssist Q, 4.6 m ID x 5 cm (PEEK)
Competitor Q, 5.0 mm ID x 5 cm
Elution:
30 min linear gradient from 0 to 1 mol/L NaCl
in 20 mmol/L sodium phosphate pH 8.0
Flow Rate:
1.0 mL/min
Detection:
UV @ 280 nm
Sample:
1) conalbumin, i) ovalbumin impurity
2) ovalbumin, 3) trypsin inhibitor
1
i
2
3
1
i
2
3
figure 3
Performance enhancement on FPLC system
figure 2
High resolution versus high throughput analysis of nucleotides
High resolution:
Column: TSKgel Q-STAT, 4.6 mm ID x 10 cm L (7 µm);
Eluent: A ) 20 mmol/L Tris-HCl (pH8.5) B) 0.5 mol/L NaCl in A (pH8.5)
Gradient: 0 to 100% B (10 min.); Flow rate: 1.5 mL/min.
Detection: UV @ 260 nm
H
igh throughput:
Column: TSKgel Q-STAT, 4.6 mm ID x 3.5 cm L (10 µm);
Eluent: A) 20 mmol/L Tris-HCl (pH8.5), B) 0.5mol/L NaCl in A (pH8.5)
Gradient: 0 to 100% B (1min.); Flow rate: 4.0 mL/min.
Detection: UV @ 260 nm
