IEC
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TOSOH BIOSCIENCE
INTRODUCTION TO TSKgel ION EXCHANGE COLUMNS
Tosoh Bioscience offers a broad line of high efficiency columns for
analysis and isolation of biomolecules by anion and cation exchange
chromatography. In either mode of Ion Exchange Chromatography (IEC),
the product line contains methacrylate-, silica- and polystyrene-based
columns. Proteins, peptides, oligonucleotides and other nucleic acid
fragments are typical samples that are analyzed or isolated on TSKgel
ion exchange columns. Most of the available chemistries are offered in
analytical as well as semi-preparative column formats. Particle sizes
range from 2.5 µm, for fast quality control and process monitoring, to 20
µm and larger particle sizes utilized in process scale separations.
TSKgel STAT
®
columns are the latest addition to the IEC column line.
They are designed for high effiency separation of biomolecules and low
molecular weight compounds. TSKgel STAT columns provide superior
performance at reduced analysis time. The STAT series encompasses
a range of high efficiency anion and cation exchange columns, suitable
for various applications from research to quality control.
Also available are a series of ion exchange columns based on a
polystyrene matrix. They are most suitable for analyzing small molecular
weight sugars, amino acids, individual nucleic acids, and small drug
candidates.
Packing Materials and Chemistries
Methacrylate, silica, and polystyrene are used as matrices for the
TSKgel line of ion exchange columns. The methacrylate backbone
chemistry provides a robust, hydrophilic particle that is suitable as a
support for high performance analytical and preparative separations of
biomolecules.
The polymethacrylate base resin, G5000PW (5PW), is a 10 µm spherical
particle with approximately 100 nm pores. The base resin is derivatized
either with diethylaminoethyl (DEAE), sulfopropyl (SP) or carboxymethyl
(CM) functionalities to provide a weak anion, a strong cation, and
a weak cation exchanger, respectively. While these chemistries
result in standard ion exchangers, the chemistry employed in the
manufacturing of TSKgel SuperQ-5PW results in a higher capacity
strong anion exchanger by introducing polyamine functional groups.
Due to the higher density of anion exchange sites, TSKgel
SuperQ-5PW has a smaller effective pore size than TSKgel DEAE-5PW.
For more detailed information, please refer to our
TSKgel IEC brochure
on
or request a printed copy at
FEATURES
BENEFITS
BioAssist columns
High capacity even for larger proteins (1 million Da)
Fewer runs to collect required sample amounts
Unique pore structure provides fast mass transfer
Sharper peaks improve analysis and isolation
Biocompatible PEEK column hardware
Less sample loss due to adsorption
Available in analytical and semi-prep formats
Easy scale-up
Polymer-Based Ion Exchange columns
Methacrylate backbone
Mechanically and chemically stable (pH 2-12)
Withstands repeated cleaning with base, and use of organic
solvents, denaturants and surfactants
Large pore size (100 nm) (excl. limit for proteins ~ 5,000,000 Da)
Use same column for most biopolymers
Non porous resin-based (STAT and NPR) columns
Fast QC analysis and process monitoring
Several columns available in 2 mm ID format
Reduced solvent consumption and analysis time
Silica-Based Ion Exchange columns
Smaller pore size (2SW = 12.5 nm and 3SW = 25 nm)
Most suitable for analysing smaller MW samples such as
nucleotides, drug candidates, catecholamines and small
peptides or proteins
