sec
SEC
33
CALIBRATION CURVES FOR TSKgel H-TYPE GEL PERMEATION COLUMNS
Calibration curves for TSK-GEL H
XL
columns
with polystyrene standards
Elution Time (min)
Column size:
Sample:
Eluent:
Flow Rate:
Temperature:
7.8 mm ID x 30 cm L
polystyrene standards
THF
1.0 mL/min
25°C
Molecular weight (Da)
10
2
G2500H
XL
G3000H
XL
G5000H
XL
GMH
XL
G6000H
XL
10
3
10
7
10
6
10
5
10
4
11
5
7
9
G1000H
XL
G2000H
XL
G4000H
XL
G7000H
XL
Detection:
UV @ 254 nm
3
GMH -L
XL
10
1
5
6
7
8
9
10
11
12
L
N
M
H
5
6
7
8
9
10
11
10
6
10
5
10
4
10
3
10
2
H. GMH
HR
-H
M. GMH
HR
-M
N. GMH
HR
-N
L. GMH
HR
-L
Calibration curves for TSK-GEL H
HR
columns with polystyrene standards
Column:
TSKgel H
HR
series (7.8 mm ID x 30 cm L)
Elution:
TH
Sample:
polystyrene standards
F
Flow Rate:
1.0 mL/min
Temperature: 25°C
Detection:
UV @ 254 nm
10
6
10
5
10
4
10
3
10
2
Molecular weight (Da)
a
b
c
d
e
f
g
h
a. G1000H
HR
b. G2000H
HR
c. G2500H
HR
d. G3000H
HR
e. G4000H
HR
f. G5000H
HR
g. G6000H
HR
h. G7000H
HR
Elution Volume (mL)
Molecular weight (Da)
Elution Volume (mL)
10
7
1.5
2.0
2.5
3.0
3.5
Molecular weight
Calibration curves for TSK-GEL SuperH columns with polystyrene standards
Column:
TSK-GEL SuperH series (6.0 mm ID x 15 cm L)
Eluent:
THF
Flow rate:
0.6 mL/min
Temperature: 25°C
Detection:
UV@254 nm
Sample:
polystyrene standards
Elution volume (mL)
10
6
10
5
10
4
10
3
10
2
1
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
SuperH7000
SuperH6000
SuperH5000
SuperH4000
SuperH3000
SuperH2500
SuperH2000
SuperH1000
10
7
1.5
2.0
2.5
3.0
Molecular weight
Elution volume (mL)
10
6
10
5
10
4
10
3
10
2
1
2
3
4
SuperHM-H
SuperHM-M
SuperHM-N
SuperHM-L
1
2
3
4
10
1
10
3
10
5
10
7
1
2
3
4
5
6
SuperHZ1000
SuperHZ2000
SuperHZ2500
SuperHZ3000
SuperHZ4000
Elution volume (mL)
Molecular weight
Calibration curves for TSK-GEL SuperHZ columns with polystyrene standards
Column:
TSK-GEL SuperHZ series (4.6 mm ID x 15 cm L)
Eluent:
THF
Flow rate:
0.35 mL/min
10
2
10
3
10
4
10
5
10
6
10
7
10
8
SuperHZM-N
SuperHZM-M
SuperHZM-H
Molecular weight
Temperature: 25°C
Sample:
standard polystyrene
Inj. volume:
2 µL
Elution volume (mL)
1
2
3
4
5
1
2
3
4
5
1
2
3
1
2
3
1
2
3
4
5
Column size: 7.8 m ID x 30 cm L; Sample: polystyrene
standards; Eluent: THF; Flow rate: 1.0 mL/min;
Temp.: 25°C; Detection: UV @ 254 nm
10
1
10
2
10
3
10
4
10
5
10
6
10
7
10
8
1.5
2.5
3.5
4.5
5.5
6.5
Elution time (minutes)
M
W
TSKgel SuperMultiporeHZ-N
TSKgel SuperMultiporeHZ-M
TSKgel SuperMultiporeHZ-H
Columns:
TSKgel SuperMultiporeHZ-N,
3µm, 4.6mm ID x15cm
TSKgel SuperMultiporeHZ-M,
4µm, 4.6mm ID x15cm
TSKgel SuperMultiporeHZ-H,
6µm, 4.6mm ID x15cm
Eluent:
THF
Flow rate:
0.35mL/min
Temp:
25°C
Detection:
UV@254nm (UV-8020 w/ 2.4µL cell)
Sample:
PStQuick polystyrene standards
Dim
Mo
Flo
Det
Tem
Sa
Calibration curves for TSKgel SuperMultiporeHZ-M,
H and N columns
Calibration curves for TSKgel SuperHZ columns with polystyrene standards
Calibration curves for TSKgel SuperH columns with polystyrene standards
Calibration curves for TSKgel H
HR
columns with polystyrene standards
Calibration curves for TSKgel H
XL
columns with poly-
styrene st ndards
Column: TSKgel SuperH series (6.0 mm ID x 15 cm L); Eluent: THF; Flow rate: 0.6 mL/min;
Temp.: 25°C; Detection: UV @ 254 nm; Sample: polystyrene standards
Column: TSKgel SuperHZ series (4.6 mm ID x 15 cm L); Eluent: THF; Flow rate: 0.35 mL/min;
Temp.: 25°C; Sample: polystyrene standards; Inj. volume: 2 μL
Columns: TSKgel SuperMultiporeHZ-N, 3 μm, 4.6 mm ID x
15 cm L, TSKgel SuperMultiporeHZ-M, 4 μm, 4.6 mm ID x
15 cm L, TSKgel SuperMultiporeHZ-H, 6 μm, 4.6 mm ID x
15 cm L; Mobile phase: THF; Flow rate: 0.35 mL/min;
Detection: UV @ 254 nm; Temp.: 25°C;
Samples: polystyrene standards
Column: TSKgel H
HR
se ies (7.8 mm ID x 30 cm L); Sample: polystyrene standards; Elution: THF
Flow rate: 1.0 mL/min; Temp.: 25°C; Detection: UV @ 254 nm
The best results are obtained when
selecting a column with the sample’s
molecular weight in the linear portion of
the calibration curve.
