SEC
32
TSKgel H
XL
, H
HR
, SuperH AND SuperHZ GEL PERMEATION COLUMNS
Polymer-based columns for Gel Permeation Chromatography of organic-soluble polymers
Series Type
SuperMultiporeHZ
SuperHZ
H
XL
SuperH
H
HR
Application focus
Ultra-high performance
with a low dead
volume and a wide
pore distribution in
each particle for
superior linearity
High-throughput polymer
analysis with ultra low
polymer adsorption.
Limited solvent
compatibility range.
Conventional polymer
analysis with ultra low
polymer adsorption. Ltd
solvent compatibility
range.
High-throughput
polymer
analysis with
expanded solvent
compatibility.
Conventional
polymer
analysis with
expanded solvent
compatibility
range.
Particle size
3, 4 and 6 µm,
depending on pore size
3, 5 and 10 µm,
depending on pore size
5, 9 and 13 µm,
depending on pore
size
3 and 5 µm,
depending on pore
size
5 µm
Theoretical plates
1
20,000/15 cm column 16,000/15 cm column
16,000/30 cm column 16,000/15 cm
column
16,000/30 cm
column
Maximum
temperature
60°C
G1000 - G4000 60°C
G5000 - mixed 80°C
G1000 - G4000 60°C
G5000 - mixed 80°C
140°C
140°C
220°C for H
HR
HT2
Standard shipping
solvent
THF
THF
THF
2
THF
2
THF
2
THF can be switched
to
none
benzene, chloroform, toluene, xylene,
dichloromethane
3
and dicholoroethane
3
see our website for detailed
information
Other shipping
solvents available?
yes
4
yes
4
no
Number of solvent
substitutions
-
One time only
One time only
Several
5
Several
5
Solvent exchange
instructions
Linear gradient with a
2 %/min rate of change
at a flow rate <0.25 mL/
min
Linear gradient with a
2 %/min rate of change
at a flow rate <0.5 mL/
min
Linear gradient with a 2%/min rate of
change according to flow rates listed
on our website
1) Theoretical plates listed are based on smallest particle size listed
2) High-temperature columns (HT) are shipped with OCDB (Orthochlorodivinylbenzene) as standard shipping
solvent.
3) Switching from THF to dichloromethane and dichloroethane is not recommended for G1000 pore size
columns.
4) See our website for available shipping solvents
5) After switching to a very polar solvent such as acetone,
switching back to a nonpolar solvent is not recommended.
HIGHLIGHTS
Porous, highly cross-linked, spherical polystyrene divinylbenzene
(PS-DVB) resin.
Five different TSKgel H-type columns are available
Expanded molecular weight ranges with exclusion limits from 1,000
g/mol to an estimated 4 x 10
8
g/mol
Minimal shrinking and swelling of the column bed
Chemically and thermally stable
Semi-micro SuperMultiporeHZ, SuperHZ and Super H columns for
reduced solvent consumption in high throughput analysis
Multipore columns provide linear calibration curves over a wider
MW range for conventional GPC (Multipore H
XL
) and semi-micro
GPC (SuperMultiporeHZ)
Mixed bed GPC columns for ultra-high temperature GPC up to 220°C
TSKgel H Series columns are recommended for the analysis of organic-
soluble polymers and are packed with spherical particles composed
of polystyrene cross-linked with divinylbenzene (PS-DVB). Each line
of columns within this series differs in degree of inertness and operat-
ing temperature range. The packings are available in eight pore sizes
and span four different column chemistries. For polymer samples with
a broad molecular range, packing of several pore sizes are provided
in the mixed bed columns: TSKgel SuperHZM series, TSKgel SuperHM
series, TSKgel GMH
XL
, TSKgel GMH
HR
, and selected high temperature
versions provide linear calibration curves up to several million Daltons
(see page 53).
Column Selection
Best results are obtained when selecting a column with the sample’s
molar mass in the linear portion of the calibration curve. The Super
prefix refers to the efficiency of the column. The Super series
columns contain ultra efficient particles as small as 3 µm, housed in
15 cm length columns. The smaller particle allows for equivalent
resolution to conventional H
XL
columns, with 50% less run time due to
the shorter column length. The Super series columns are an excellent
choice for high throughput polymer analysis.
table V
