87
rpc
RPC
FAST RP COLUMNS TSKgel ODS-140HTP
HIGHLIGHTS
Moderate pressure at high flow rates
High resolution and high efficiency
High throughput applications
Compatible with HPLC and UPLC systems
Moderate carbon content
Polylayer bonding chemistry
TSKgel ODS-140HTP
columns were developed for use in high throughput
applications, including drug discovery, pharmacokinetics and peptide
digest separations. They are packed with 2.3 µm particles, providing high
resolution and short analysis times at moderate pressure. The lower
pressure drop reduces the burden on the hardware, allowing TSKgel
ODS-140 HTP columns to be used with either UHPLC or conventional
HPLC systems. The backpressure of this columns is less than half of
the pressure of a sub-2 µm column of the same dimensions
(Figure 10)
.
APPLICATIONS
In Vietnamese and Chinese traditional medicine, hot aqueous extract
of Crinum latifolium is used because of its antitumor activity. Crinum
latifolium is thought to possess antiviral and immunostimulative
properties and shows immunomodulatory properties in human
peripheral blood mononuclear cells. The analysis of products derived
from plant extracts is a challenging chromatographic task. Due to the
high number ob components the column needs to provide a high peak
capacity, as shown in
Figure 11
.
figure 11
Analysis of crinum latifolium
figure10
Column backpressure versus particle size
Column: TSKgel ODS-140HTP 2.3 μm (2.0 mm ID x 5.0 cm, 10 cm L)
Sub-2 μm ODS columns (2.1 mm ID x 5.0 cm L); Eluent: H
2
O/CH
2
CN - 50/50
0
2
4
6
8
10
12
14
16
Retention time (minutes)
Detector response (AU)
Column: TSKgel ODS-140HTP 2.3 µm, 2.1 mm ID x 10 cm L;
Sample: Crinum latifolium L extract, 2 µl; Eluent: A: water, B: acetonitrile;
Gradient: 0 min (5% B), 1.2 min (5 % B), 4 min (30 % B), 15 min (68 % B),
15.1 min (100 % B), 20min (100% B); Flow rate: 0.4 mL/min; Temp.: 40°C;
Detection: UV@220 nm; Sampling rate: 80 Hz
Part #
Description
ID Length
Particle
Pore
Number
Maximum
(mm)
(cm)
size (µm)
size (nm)
theoretical
pressure
plates
drop (MPa)
TSKgel stainless steel columns
21927
TSKgel ODS-140HTP
2.1
5.0
2.3
14
≥
7,000
60.0
21928
TSKgel ODS-140HTP
2.1
10.0
2.3
14
≥
14,000
60.0
ORDERING INFORMATION
