91
rpc
RPC
Polymer based rp columns TSKgel Octadecyl-NPR / -2PW / -4PW/ -Phenyl-5PW RP
HIGHLIGHTS
Polymer-based RPC columns are chemically
stable
at pH 2-12, allowing operation at basic pH where
silica-based columns have limited chemical stability.
Polymer-based columns can be cleaned and impurities removed by
using either strong acid or base.
Non-porous resins (NPR) or porous resins of various pore sizes
available. Column selection is based on sample MW or application.
2.5 µm particle size TSKgel Octadecyl-NPR resin features fast
kinetics resulting in high column efficiency and quantitative protein
recovery at sub-microgram loads.
TSKgel Octadecyl-2PWwith 5 µm particle size and 12.5 nm pores size.
TSKgel Octadecyl-4PW with 7 µm particle size and 50 nm pores size.
TSKgel Phenyl-5PW with 10 µm particle size and an average pore
size of 100 nm. In comparison with the Phenyl-5PW packing material
used in HIC, the greater level of hydrophobicity in TSKgel Phenyl-
5PW RP makes this material more suitable for use in RPC.
APPLICATIONS
TSKgel OCTADECYL-NPR
High efficiency purification of proteins and peptides at sub-
microgram loads
Stable to higher pressures than porous particles
Improved recovery at low sample concentration over traditional
porous resins
TSKgel OCTADECYL-2PW
For analyzing small MW pharmaceutical compounds at basic pH
Faster analysis than competitive polymeric RPC columns
TSKgel OCTADECYL-4PW
Recommended for peptides and small proteins
TSKgel PHENYL-5PW RP
Ideal for the separation of proteins, including high MW
Able to handle high loads (high capacity)
ORDERING INFORMATION
Part #
Description
ID
Length
Particle
Number
Flow rate (mL/min)
Maximum
(mm)
(cm)
size (µm)
theoretical
range
pressure
plates
drop (MPa)
TSKgel stainless steel columns
0014005 Octadecyl-NPR nonporous
4.6
3.5
2.5
≥
1,000
1.0 - 1.5
20.0
0018754 Octadecyl-2PW, (10 - 12.5 nm)
2.0
15.0
5
≥
5,000
0.07 - 0.11
7.0
0017500 Octadecyl-2PW, (10 - 12.5 nm)
4.6
15.0
5
≥
6,000
0.4 - 0.6
10.0
0017501 Octadecyl-2PW, (10 - 20 nm)
6.0
15.0
5
≥
6,000
0.5 - 1.0
10.0
0018755 Octadecyl-4PW, 50 nm
2.0
15.0
7
≥
2,000
0.08 - 0.17
10.0
0013351 Octadecyl-4PW, 50 nm
4.6
15.0
7
≥
2,000
0.5 - 1.0
12.0
0016257 Octadecyl-4PW, 50 nm
21.5
15.0
13
≥
2,000
3.0 - 6.0
2.5
0018756 Phenyl-5PW RP, 100 nm
2.0
7.5
10
≥
400
0.05 - 0.1
1.0
0008043 Phenyl-5PW RP, 100 nm
4.6
7.5
10
≥
500
0.5 - 1.0
3.0
0016260 Phenyl-5PW RP, 100 nm
21.5
15.0
13
≥
1,000
6.0 - 8.0
3.0
Glass columns
0014007 Phenyl-5PW RP Glass, 100 nm
8.0
7.5
10
≥
700
1.0 - 2.0
2.0
Guard column products
0019007
Phenyl-5PW RP Cartridge, pk 3 *
3.2
1.5
10
For P/N 0008043
0017502 Octadecyl-2PW Guard column
4.6
1.0
5
For P/N 0017500
0017503 Octadecyl-2PW Guard column
6.0
1.0
5
For P/N 0017501
0019008 Octadecyl-4PW Cartridge, pk 3
*
3.2
1.5
7
For P/N 0013351
0019308
Guard cartridge holder
2.0
1.0
For all 2 mm ID cartridges
0019018 Guard cartridge holder
3.2
1.5
For 4.6 mm ID Octadecyl 4-PW and Phenyl-5PW RP
columns
*needs cartridge holder
