88
RPC
HIGHLIGHTS
The silica particles used in Super series columns are monodisperse
spherical 2.3 µm beads with 11 nm (110 Å) pores
TSKgel Super-ODS, Super-Octyl and Super-Phenyl packings are
bonded with, respectively, C18, C8 and phenyl functional groups.
The bonded phases have a polymeric structure. An exhaustive
endcapping reaction minimizes the presence of residual silanol
groups
2 µm particles provide superior resolution and speed, as well as
improved sensitivity
Pressure drop is not excessive due to the monodisperse particle
size distribution
APPLICATIONS
TSKgel Super-ODS, Super-Octyl, Super-Phenyl
Recommended for small molecular weight compounds (<10,000 Da) such
as peptides, amino acids, tryptic digests, nucleotides, pharmaceutical
molecules, and food and beverage samples.
Optimizing Results with Fast RP Columns
Super series columns can be used on a regular HPLC system if the
dead volume is minimized, although optimal results are obtained with an
UHPLC system.
The following recommendations are for 4.6 mm ID columns. Use propor-
tionately lower values for 2 mm ID columns.
1.
A guard filter is highly recommended to reduce particulate
contamination from the sample or system components.
2.
Keep sample volume less than 10 µL.
3.
To ensure minimal extra-column volume, keep tubing as short as
possible (extra-column volume less than 5 µL between column and
detector).
4.
Conventional 0.1 mm ID connecting tubing may be used
(0.005).
5.
The smallest detector time constant should be selected
(if possible, less than 50 ms).
6.
The detector flow cell should be 2 µL or less for best results. A
standard HPLC flow cell (10 µL) can be used as an alternative,
however, it is recommended that the heating coil is removed.
Fast RP Columns TSKgel Super-ODS / Super-Octyl / Super-Phenyl
Part #
Description
ID
Length
Particle Number
Flow rate (mL/min)
Maximum
(mm)
(cm)
size (µm) theoretical
range
pressure
plates
drop (MPa)
TSKgel Stainless Steel Columns
0020015 Super-ODS, 11 nm
1.0
5.0
2.3
≥
15,000
0.03 - 0.05
15.0
0019541 Super-ODS, 11 nm
2.0
5.0
2.3
≥
6,000
0.15 - 0.2
25.0
0019542 Super-ODS, 11 nm
2.0
10.0
2.3
≥
12,000
0.15 - 0.2
25.0
0018154 Super-ODS, 11 nm
4.6
5.0
2.3
≥
8,000
1.0 - 2.5
30.0
0018197 Super-ODS, 11 nm
4.6
10.0
2.3
≥
16,000
1.0 - 2.5
30.0
0020013 Super-Octyl, 11 nm
2.0
5.0
2.3
≥
15,000
0.15 - 0.20
15.0
0020014 Super-Octyl, 11 nm
2.0
10.0
2.3
≥
1,500
0.15 - 0.20
30.0
0018275 Super-Octyl, 11 nm
4.6
5.0
2.3
≥
8,000
1.0 - 2.5
30.0
0018276 Super-Octyl, 11 nm
4.6
10.0
2.3
≥
16,000
1.0 - 2.5
30.0
0020017 Super-Phenyl, 11 nm
2.0
5.0
2.3
≥
3,000
0.15 - 0.20
8.0
0020018 Super-Phenyl, 11 nm
2.0
10.0
2.3
≥
6,000
0.15 - 0.20
15.0
0018277 Super-Phenyl, 11 nm
4.6
5.0
2.3
≥
8,000
1.0 - 2.5
30.0
0018278 Super-Phenyl, 11 nm
4.6
10.0
2.3
≥
16,000
1.0 - 2.5
30.0
Guard column products
0019672 Guard cartridge, pk 3*
2.0
1.0
2.3 For 2 mm ID Super-ODS columns
0019308 Cartridge holder
For P/N 0019672
0018207 Guard filter, pk 3*
4.0
0.4
For 4.6 mm ID columns (Super-ODS, -Octyl, -Phenyl)
0018206 Guard filter holder
4.0
0.4
For P/N 0018207
ORDERING INFORMATION
*needs cartridge holder
