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What’s new
What’s new
TOYOPEARL AF-rProtein A HC-650F
TOYOPEARL AF-rProtein A HC-650F is the latest addition
to the TOYOPEARL line of affinity media for immunoglob-
ulin purification. HC standing for high capacity indicates
that this resin has a superior dynamic binding capacity for
human IgGs. It was designed to provide a considerably
higher binding capacity than all other commercially avail-
able Protein A resins. Typical dynamic binding capacities
for monoclonals reach 70 g/L at 5 minutes residence time.
The capacity is not diminished by feedstock titers up to
10 g/L allowing for constant process flow rates. The alka-
line stable, recombinant protein A ligand is coupled to the
renowned TOYOPEARL polymeric base particle by multi-
point-attachment in order to minimize protein A leaching.
TOYOPEARL MX-Trp-650M
TOYOPEARL MX-Trp-650M is a new multimodal cation
exchange resin with unique selectivity and high recovery.
It provides high protein binding capacities and tolerates
high conductivity feedstocks. TOYOPEARL MX-Trp-650M
is based on a rigid methacrylic polymer matrix, which
provides excellent pressure/flow properties and allows
high flow rates at large scale. Its ligand, the amino acid
tryptophan, provides ionic and hydrophobic interactions.
This new mixed-mode resin is well-suited for intermediate
and polishing steps, such as aggregate removal in antibody
purification, and for the purification of targets that are diffi-
cult to purify using common purification platforms.
TOYOPEARL GigaCap DEAE-650M
TOYOPEARL GigaCap DEAE-650 is the newest member of
the renowned TOYOPEARL GigaCap series of high capacity
ion exchange media. The polymeric base bead was chemi-
cally modified to provide a high number of anionic binding
sites for increased resin capacity. The typical dynamic
binding capacity (DBC) of over 150 g/L for bovine serum
albumin exceeds the DBC of all other commercial available
DEAE chromatography media. The resin was proved to
be stable for more than 100 cleaning-in-place (CIP) cycles
with 0.5 M NaOH. Good mass transfer kinetics enable the
resin to maintain DBC at faster linear velocities and leads
to a narrow elution peak. This results in smaller and more
concentrated in-process pool volumes, thus reducing the
amount of water and buffer needed. The high capacity
and low back pressure creates opportunities for increased
throughput in various anion exchange purification steps
and is ideally suited for efficient plasma protein purification.
In addition to the new DEAE functionality the TOYOPEARL
GigaCap series was also extended by small particle
(S-grade, 35 µm) versions of the strong ion exchangers
TOYOPEARL GigaCap Q and S.
What’s New
