4
introduction
Introduction
comparison of prepacked columns with respEctive bulk polymeric media
MODE TSKgel column
ToyoScreen
®
PROCESS
BULK POLYMERIC MEDIA FOR SCALE-UP AND
FOR ANALYSIS
DEVELOPMENT COLUMNS PRODUCTION
SEC
TSKgel G1000PW - G6000PW
TOYOPEARL HW-40 - HW-75
IEC
TSKgel SP-5PW
ToyoScreen SP-650M
TSKgel SP-5PW TOYOPEARL SP-650S, M or C
ToyoScreen SP-550C
TOYOPEARL SP-550C
ToyoScreen MegaCap
®
II SP-550EC
TOYOPEARL MegaCap II SP-550EC
ToyoScreen GigaCap
®
S-650M
TOYOPEARL GigaCap S-650M
ToyoScreen GigaCap CM-650M
TOYOPEARL GigaCap CM-650M
TSKgel CM-5PW
ToyoScreen CM-650M
TOYOPEARL CM-650S, M or C
TSKgel DEAE-5PW
ToyoScreen DEAE-650M TSKgel DEAE-5PW TOYOPEARL DEAE-650S, M or C
TSKgel SuperQ-5PW
ToyoScreen SuperQ-650M TSKgel SuperQ-5PW TOYOPEARL SuperQ-650S, M or C
ToyoScreen GigaCap Q-650M
TOYOPEARL GigaCap Q-650M
ToyoScreen GigaCap DEAE-650M
TOYOPEARL GigaCap DEAE-650M
ToyoScreen QAE-550C
TOYOPEARL QAE-550C
ToyoScreen Q-600C AR
TOYOPEARL Q-600C AR
HIC
TSKgel Ether-5PW
ToyoScreen Ether-650M TSKgel Ether-5PW TOYOPEARL Ether-650S, M
TSKgel Phenyl-5PW
ToyoScreen Phenyl-650M TSKgel Phenyl-5PW TOYOPEARL Phenyl-650S, M or C
ToyoScreen Phenyl-600M
TOYOPEARL Phenyl-600M
ToyoScreen Butyl-650M
TOYOPEARL Butyl-650S, M or C
ToyoScreen Butyl-600M
TOYOPEARL Butyl-600M
ToyoScreen Hexyl-650C
TOYOPEARL Hexyl-650C
ToyoScreen PPG-600M
TOYOPEARL PPG-600M
ToyoScreen SuperButyl-550C
TOYOPEARL SuperButyl-550C
MX
ToyoScreen MX-Trp-650M
TOYOPEARL MX-Trp-650M
AFC
TSKgel Chelate-5PW
ToyoScreen AF-Chelate-650M
TOYOPEARL AF-Chelate-650M
TSKgel Tresyl-5PW
TSKgel Tresyl-5PW TOYOPEARL AF-Tresyl-650M
ToyoScreen AF-Blue HC-650M
TOYOPEARL AF-Blue HC-650M
ToyoScreen AF-Red-650M
TOYOPEARL AF-Red-650M
ToyoScreen AF-HeparinHC-650M
TOYOPEARL AF-HeparinHC-650M
ToyoScreen AF-rProtein A-650F
TOYOPEARL AF-rProtein A-650F
ToyoScreen AF-rProtein A HC-650F
TOYOPEARL AF-rProtein A HC-650F
table I
TSKgel resins
The polymeric resins with particle sizes of 20 µm and 30 µm
used in TSKgel columns are also available in bulk quantities
for large scale ion exchange and hydrophobic interaction
chromatography. Their mechanical stability and perme-
ability make them excellent for use when increased separa-
tion performance and plate count are needed for optimum
preparative or process chromatography.
TOYOPEARL resins
TOYOPEARL resins are hydrophilic macroporous meth-
acrylic resins for large-scale chromatographic applications.
Their rigid polymeric backbone has better pressure-flow
properties than most other commercially made materials.
Therefore, higher linear operating velocities can be used for
faster process throughput and decreased recycling times.
TOYOPEARL resins are stable over the pH 2-12 range for
normal operating conditions and pH 1-13 for cleaning condi-
tions. The resins are available in average particle sizes of 35
µm, 65 µm, 75 µm, and 100 µm for high resolution, interme-
diate purification, or capture chromatography.
TOYOPEARL resins are also offered in many different pore
diameters for size exclusion, ion exchange, hydrophobic
interaction, and affinity chromatography. Pore diameter
and surface area can be optimized to ensure excellent
kinetic access and binding capacity of your target regard-
less of molecular size.
For predictable results in scale-up, TOYOPEARL resins are
based on the same chemistries as the prepacked TSKgel
columns. This allows the seamless direct scale-up of
methods developed on TSKgel columns to TOYOPEARL
resins.
