14
SEC
Properties in organic eluents
TOYOPEARL resins can be used in organic solvents or
mixtures of organic solvents and water. Bed volumes may
swell or shrink relative to water depending on the solvent
as shown in Tables III and IV. DMSO can be used for SEC
of oligosaccharides and polyethylene glycols. The compati-
bility of DMF with TOYOPEARL also permits SEC separation
of hydrophobic substances such as polystyrenes.
Other applications
The TOYOPEARL HW-type resins are commonly used in
size exclusion chromatography and desalting applications.
Some other important uses of these materials are:
Removal of surfactants such as Triton
®
X-100 from
biological solutions by an adsorption mechanism
Use in hydrophobic interaction chromatography (HIC)
for the separation of very hydrophobic molecules
Use in HIC separations as a guard column for
hydrophobic impurities
Possible use as a stationary phase for either normal or
reversed phase separations depending on solvent
system selected
ToyoScreen RoboColumns are miniaturized chromato-
graphic columns for operation with a robotic liquid
handling system, such as the Freedom EVO
®
from TECAN.
This approach allows automated high-throughput, small-
scale biochromatographic separations of protein samples
by running up to eight individual columns simultaneously.
ToyoScreen RoboColumns packed with the most popular
TOYOPEARL SEC/IEC/HIC media are available with 200 µL
and 600 µL resin volumes.
size exclusion
chromatography
table III
Additional swelling data for TOYOPEARL HW-40
TOYOPEARL DMSO Ethyl
Benzene CHCl
3
CHCl
3
/
Acetate
MeOH (1:1)
HW-40
140
80
70
105
120
table IV
Swelling properties in various solvents
TOYOPEARL
HW-40 HW-50 HW-55 HW-65 HW-75
Water
100
100
100
100
100
0.2 mol/L KCl
100
100
100
100
100
MeOH
100
100
100
100
105
EtOH
100
100
100
100
110
DMF
110
110
105
105
120
Acetone
80
80
85
90
110
Toluene
65
70
70
75
90
ORDERING INFORMATION
TOYOPEARL LabPak
Part # Product description
Container size (mL)
Particle size (
µ
m)
0019821 SECPAK LMW (HW-40F, HW-50F, HW-55F)
3 x 150
30 - 60
0019819 SECPAK HMW (HW-55F, HW-65F, HW-75F)
3 x 150
30 - 60
0019820 SECPAK HP (HW-40S, HW-50S, HW-55S, HW-65S)
4 x 150
20 - 40
