22
IEC
Figures 5, 6 and 7 show the breakthrough curves for the
three TOYOPEARL GigaCap resins. They are compared
where possible with the most current equivalent competi-
tive resin. Each trace shows the dynamic binding capacity of
the resin up to 10% breakthrough plus the elution profile for
the target molecule. Please note the significant reduction
in elution pool volumes of the TOYOPEARL GigaCap resins
when compared to other products.
The concentration of the eluted peak is proportionally
increased as well. It is possible to achieve reductions in
elution pool volumes of over 75%. This can reduce the cost
of further downstream process steps.
The strong ion exchange resins TOYOPEARL GigaCap S
and GigaCap Q are now also available with smaller particle
size (S-grade, 35 µm), providing higher resolution for better
separaion of process impurities.
Ion exchange
chromatography
figure 5
Time (min.)
-200
0
200
400
600
800
1000
1200
75 95
115
135
155
175
195
215
mAu (280nm)
Toyopearl GigaCap S-650M (hlgG)
Competitor S (h-lgG)
At 5% breakthrough value:
GigaCap S elutes in 8.3 CVs
Competitor S elutes in 39 CVs
Toyopearl GigaCap S-650M vs. Competitor S elution pool
volume comparison
Column size:
6 mm ID × 40 mm bed
Sample:
polyclonal human IgG (1 mg/mL)
Loading Buffer: 0.1 mol/L acetate buffer (pH=4.7)
Elution Buffer:
0.1 mol/L acetate buffer (pH=4.7) + 1.0 mol/L NaCl
Linear velocity: 212 cm/hr
Detection:
UV @ 280 nm
Column size: 6 mm ID x 40 mm bed; Sample: polyclonal human
IgG (1 mg/mL); Loading Buffer: 0.1 mol/L acetate buffer (pH= 4.7)
Elution Buffer: 0.1 mol/L acetate buffer (pH= 4.7) + 1.0 mol/L NaCl
i ear velocity: 212 cm/h; Detection: UV @ 280 nm
TOYOPEARL igaCap S-650M vs. Competitor S eluti
p l
i
figure 7
-200
0
200
400
600
800
1000
1200
0
50
100
150
200
250
mAu (280nm)
Time(min.)
ToyopearlGigaCap CM-650M
Toyopearl GigaCap CM-650M elution pool volume
Column size:
6mm ID x 40mm
Sample:
polyclonal human IgG (1 mg/mL)
Loading buffer:
50 mmol/L sodium acetate buffer (pH=4.7)
Elution buffer:
50 mmol/L sodium acetate buffer (pH=4.7)
+ 0.5 mol/L NaCl
Linear velocity: 212 cm/hr
Detection:
UV @ 280 nm
GigaCap CM elutes in 24 CVs
Column size: 6 mm ID x 40 mm L; Sample: polyclonal human IgG
(1 mg/mL); Loading buffer: 50 m ol/L sodium acetate buffer (pH= 4.7);
El ti ffer: 15 mmol/L Tris-HCl (pH= 8.7) + 1.0 mol/L NaCl
Linear velocity: 212 cm/h; Detection: UV @ 280 nm
TOYOPEARL GigaCap CM-650M elution pool volume
figure 6
0
200
400
600
800
1000
1200
0
50
100
150
200
mAu (280 nm)
Time (min.)
At 5% breakthrough value:
GigaCap Q-650M = 13 CVs
Competitor Q = 48 CVs
Toyopearl GigaCap Q-650M (hlgG)
Competitor Q (h-lgG)
Toyopearl GigaCap Q-650M vs. Competitor Q elution
pool volume comparison
Column size:
6 mm ID x 40 mm
Sample:
polyclonal human IgG (1 mg/mL)
Loading buffer 15 mmol/LTris-HCl (pH=8.7)
Elution buffer:
15 mmol/L Tris-HCl (pH=8.7) + 1.0 mol/L NaCl
Linear velocity: 212 cm/hr
Detection:
UV @ 280 nm
Column size: 6 mm ID x 40 mm L; Sample: polyclonal human IgG
(1 mg/mL); Loading buffer: 15 mmol/LTris-HCl (pH= 8.7);
Elution buffer: 15 mmol/L Tris-HCl (pH= 8.7) + 1.0 mol/L NaCl;
Linear velocity: 212 cm/h; Detection: UV @ 280 nm
TOYOPEARL GigaC p Q-650M vs. Competitor Q elution pool
volume co parison
