21
IEC
Ligand Attachment technology
Tosoh Bioscience applies three different ligand attach-
ment chemistries reflecting three generations of ligand
attachment technology. The “traditional” method, or first
generation of attaching the ion exchange ligand, is directly
to the resin surface through a proprietary spacer arm.
TOYOPEARL and TSKgel PW type ion exchange resins
using this traditional bead functionalization method are:
TOYOPEARL SP-650 and SP-550
TSKgel SP-3PW and SP-5PW
TOYOPEARL CM-650
TOYOPEARL Q-600C AR
TOYOPEARL Q-550
TOYOPEARL DEAE-650
TSKgel DEAE-5PW
TOYOPEARL MegaCap II SP-550EC
A second generation attachment chemistry (Type A noted in
Table IV) for increasing protein binding within the accessible
surface area, is to add a carbon spacer network between
the bead surface and the ligand. It is also possible to attach
ligand groups along the length of the spacer network thus
improving capacity. There are two resins which incorpo-
rates this type A ligand attachment chemistry:
TOYOPEARL SuperQ-650
TSKgel SuperQ-5PW
A third generation ligand attachment chemistry (Type B
noted in Table IV) improves the accessible location of the
ligand groups. The result of this modification is signifi-
cantly increased capacity and improved mass transfer.
Improved mass transfer also reduces the target molecule
elution volume. All TOYOPEARL GigaCap resins use this
Type B ligand attachment chemistry:
TOYOPEARL GigaCap S-650
TOYOPEARL GigaCap CM-650
TOYOPEARL GigaCap Q-650
TOYOPEARL GigaCap DEAE-650
Ion exchange
chromatography
Figure 4
Pure Product
Capture
Intermediate
Purification
Polishing
QC
Same selectivity HPLC columns
are available for most process media
Process step
Process media
75 µm
TOYOPEARL SuperQ-650C
TOYOPEARL DEAE-650C
TOYOPEARL QAE-550C
TOYOPEARL Q-600C AR
TOYOPEARL GigaCap Q-650M
TOYOPEARL GigaCap DEAE-650M
65 µm TOYOPEARL SuperQ-650M
TOYOPEARL DEAE-650M
TOYOPEARL SuperQ-650S
TOYOPEARL DEAE-650S
TOYOPEARL GigaCap Q-650S
35 µm
30 µm TSKgel SuperQ-5PW (30)
TSKgel DEAE-5PW (30)
TSKgel SuperQ-5PW (20)
TSKgel DEAE-5PW (20)
20 µm
10 µm TSKgel SuperQ-5PW
7.5 mm IDx 7.5 cm L
TSKgel DEAE-5PW
7.5 mm ID x 7.5 cm L
TOYOPEARL MegaCap II SP-550EC
TOYOPEARL SP-650C
TOYOPEARL SP-550C
TOYOPEARL CM-650C
TOYOPEARL GigaCap S-650M
TOYOPEARL GigaCap CM-650M
TOYOPEARL SP-650M
TOYOPEARL CM-650M
TOYOPEARL SP-650S
TOYOPEARL CM-650S
TOYOPEARL GigaCap S-650S
TSKgel SP-5PW
7.5 mm ID x 7.5 cm L
TSKgel CM-5PW
7.5 mm ID x 7.5 cm L
100 µm
200 µm
TSKgel SP-5PW (30)
TSKgel SP-3PW (30)
TSKgel SP-5PW (20)
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