Process DEVELOPMENT
109
BULK
TOYOPEARL HW-type resins, available in pore sizes ranging from 5 nm
to >100 nm, are employed in size exclusion chromatography (SEC).
TOYOPEARL HW-65 and HW-55 resins are used as starting materials
for the production of all other functionalized TOYOPEARL resins. The
large pore size of HW-65 (100 nm) allows unhindered access of large
proteins to the stationary phase, resulting in faster separation and
shorter recycling times.
For predictable results during scale up, TOYOPEARL resins are based on
the same chemistry as the prepacked TSKgel columns. This allows for
seamless scale up from the laboratory to manufacturing.
TSKgel Bulk Resins
TSKgel resins are larger particle size versions of the chemically
equivalent methacrylic packing of analytical-scale TSKgel columns
used for protein analysis and purification. The TSKgel resin product
line consists of DEAE-5PW, SuperQ-5PW, SP-5PW, and SP-3PW resins
for ion exchange, Tresyl-5PW resins for afffinity chromatography, and
Ether-5PW and Phenyl-5PW resins for HIC. TSKgel resins are often
employed to simplify scale-up from analytical columns, as only the
particle size is different. Their small particle sizes, high degree of cross-
linking and high mechanical stability make TSKgel resins the preferred
choice for high efficiency purifications.
Mode Resin
Grade/particle size (µm)
Pore
MW range Operating Max. pressur
e
size (nm)
**
Proteins (Da)
pH range
(MPa)
SEC
TOYOPEARL HW-40
S (20-40), F (30-60), C (50-100)
5
1 x 10
2
- 1 x 10
4
2–12
0.3
TOYOPEARL HW-50
S (20-40), F (30-60)
12.5
5 x 10
2
- 8 x 10
4
2–12
0.3
TOYOPEARL HW-55
S (20-40), F (30-60)
50
1 x 10
3
- 7 x 10
5
2–12
0.3
TOYOPEARL HW-65
S (20-40), F (30-60)
100
4 x 10
4
- 5 x 10
6
2–12
0.3
TOYOPEARL HW-75
S (20-40), F (30-60)
> 100
5 x 10
5
- 5 x 10
7
2–12
0.3
IEC
TSKgel SuperQ-5PW
20 and 30
100
< 5 x 10
6
2–12
2.0
TSKgel DEAE-5PW
20 and 30
100
< 5 x 10
6
2–12
2.0
TSKgel SP-5PW
20 and 30
100
< 5 x 10
6
2–12
2.0
TSKgel SP-3PW
30
25
< 1 x 10
4
2–12
2.0
TOYOPEARL SuperQ-650
S (20-50), M (40-90), C (50-150)
100
< 5 x 10
6
2–12
0.3
TOYOPEARL DEAE-650
S (20-50), M (40-90), C (50-150)
100
< 5 x 10
6
2–12
0.3
TOYOPEARL GigaCap Q-650
S (20-50), M (50-100)
100
< 5 x 10
6
2–12
0.3
TOYOPEARL GigaCap DEAE-650
M (50-100)
100
< 5 x 10
6
2–12
0.3
TOYOPEARL SP-650
S (20-50), M (40-90), C (50-150)
100
< 5 x 10
6
2–12
0.3
TOYOPEARL CM-650
S (20-50), M (40-90), C (50-150)
100
< 5 x 10
6
2–12
0.3
TOYOPEARL GigaCap S-650
S (20-50), M (50-100)
100
< 5 x 10
6
2–12
0.3
TOYOPEARL GigaCap CM-650
M (50-100)
100
< 5 x 10
6
2–12
0.3
TOYOPEARL QAE-550
C (50-150)
50
< 5 x 10
5
2–12
0.3
TOYOPEARL Q-600C AR
C (50-150)
75
< 2.5 x 10
6
2-12
0.3
TOYOPEARL NH
2
-750
F (30-60)
>1000
< 5 x 10
7
2–12
0.3
TOYOPEARL SP-550
C (50-150)
50
< 5 x 10
5
2–12
0.3
TOYOPEARL MegaCap II SP-550
EC (100-300)
50
<5 x 10
5
2–12
0.3
MMC
TOYOPEARL MX-Trp-650M
M (50-100)
100
< 5 x 10
6
2–12
2.0
HIC
TSKgel Ether-5PW
20 and 30
100
< 5 x 10
6
2–12
2.0
TSKgel Phenyl-5PW
20 and 30
100
< 5 x 10
6
2–12
2.0
TOYOPEARL Ether-650
S (20-50), M (40-90)
100
< 5 x 10
6
2–12
0.3
TOYOPEARL PPG-600
M (40-90)
75
< 5 x 10
6
2–12
0.3
TOYOPEARL Phenyl-600
M (40-90)
75
< 5 x 10
6
2–12
0.3
TOYOPEARL Butyl-600
M (40-90)
75
< 5 x 10
6
2–12
0.3
TOYOPEARL Phenyl-650
S (20-50), M (40-90), C (50-150)
100
< 5 x 10
6
2–12
0.3
TOYOPEARL Butyl-650
S (20-50), M (40-90), C (50-150)
100
< 5 x 10
6
2–12
0.3
TOYOPEARL Super Butyl-550
C (50-150)
50
< 5 x 10
5
2–12
0.3
TOYOPEARL Hexyl-650
C (50-150)
100
< 5 x 10
6
2–12
0.3
AFC
TSKgel Tresyl-5PW
10
100
< 5 x 10
6
2–12
1.0
TOYOPEARL AF-Chelate-650
M (40-90)
100
< 5 x 10
6
2–12
0.3
TOYOPEARL AF-rProtein A HC-650 F (30-60)
100
< 5 x 10
6
N/A
0.3
TOYOPEARL AF-rProtein A
F (30-60)
100
< 5 x 10
6
N/A
0.3
TOYOPEARL AF-Tresyl-650
M (40-90)
100
< 5 x 10
6
N/A
0.3
TOYOPEARL AF-Epoxy-650
M (40-90)
100
< 5 x 10
6
N/A
0.3
TOYOPEARL AF-Formyl-650
M (40-90)
100
< 5 x 10
6
6-9
0.3
TOYOPEARL AF-Amino-650
M (40-90)
100
< 5 x 10
6
2-12
0.3
TOYOPEARL AF-Carboxy-650
M (40-90)
100
< 5 x 10
6
2-12
0.3
TOYOPEARL AF-Red-650
M (40-90)
100
< 5 x 10
6
4-9
0.3
TOYOPEARL AF-Blue HC-650
M (40-90)
100
< 5 x 10
6
4-9
0.3
TOYOPEARL AF-Heparin HC-650
M (40-90)
100
< 5 x 10
6
5-10
0.3
** nominal values; Pore size of base matrix
TABLe I
Characteristics of TOYOPEARL and TSKgel media
process development bulk media
