112
BULK
HIGHLIGHTS
TOYOPEARL MX-Trp-650M is a multimodal cation exchange resin
It provides high binding capacity for IgG and other proteins
It tolerates high conductivity feedstocks
Target molecules elute under mild conditions in sharp peaks
Multimodal or mixed-mode chromatography expands the range of
chromatographic modes applied in biopurification. Mixed-mode media
combine ionic and hydrophobic interactions and offer new selectivities
and a higher salt tolerance than traditional ion exchange media. Mixed-
mode media can be used for direct processing of clarified feedstocks
at physiological salt concentrations as well as for intermediate and
polishing applications. The salt tolerance of the recently introduced
TOYOPEARL NH
2
-750F anion exchange resin is to a certain extent also
based on mixed-mode interactions. Nevertheless, this resin is listed in
the ion exchange section.
TOYOPEARL MX-Trp-650M is a multimodal cation exchange resin with
unique selectivity and high recovery. It provides high protein binding
capacities and tolerates high conductivity feedstocks. In addition
to ionic groups its ligand also carries hydrophobic regions. Thus,
the binding of target molecules is determined by electrostatic and
hydrophobic contributions. TOYOPEARL MX-Trp-650M is especially
suited for the purification of target molecules that are difficult to purify
using common purification platforms.
TOYOPEARL AND TSKgel BULK RESINS FOR Mixed-Mode Chromatography
figure 8
IgG binding capacity
IgG (g/L)
60
0
20
40
80
100
120
12
10
8
6
4
2
0
% breakthrough
212 cm/h
425 cm/h
637 cm/h
849 cm/h
Column: TOYOPEARL MX-Trp-650M (6 mm ID x 4 cm);
Sample: polyclonal human IgG (1 mg/mL) in 0.05 mol/L NaAc + 0.1 mol/L
sodium chloride (pH 4.7); Linear velocity: 212, 425, 637, 849 cm/h;
Detection: UV @ 280 nm
OCH
2
CNH
O
H
C COONa
N
H
CH
2
Weak cation exchange
Hydrophobic
TOYOPEARL
Product name: TOYOPEARL MX-Trp-650M
Particle size: 50-100 µm
figure 7
TOYOPEARL MX-Trp-650M STRUCTURE
