110
BULK
TOYOPEARL BULK RESINS FOR SEC
HIGHLIGHTS
Pore sizes ranging from 5 nm to >100 nm
Three particle sizes (S, F, C)
HW-40 is ideal for desalting applications
Easy to pack in semi-preparative and process scale columns
Size exclusion chromatography (SEC) is a common technique for
separating molecules based on their apparent molecular size. For
nearly twenty-five years, TOYOPEARL SEC bulk resins, with their
macroporous packings, have been used for laboratory and production-
scale biochromatography.
TOYOPEARL SEC resins are semi-rigid, spherical polymethacrylate
beads. The resins have hydrophilic surfaces due to the presence of
ether and hydroxyl groups. The numerous surface hydroxyl groups
provide attachment points for other functional groups and ligands.
Table II
provides an overview of the TOYOPEARL SEC resin product
line including corresponding molecular weight ranges of common
target samples. Calibration curves of the TOYOPEARL HW-type resins
determined with globular proteins are presented in
Figure 5
.
Ordering information for quantities <1 L is provided at the end of this
section. For larger quantities, please contact customer service at
+49 (0) 6155 70437-30
. LABPAK kits are also available in popular
combinations of TOYOPEARL media. See the page 99 for additional
information.
Applications:
proteins, peptides, amino acids, nucleic acids,
and small molecular weight molecules. Please visit our website:
for extensive data on applications.
Column: 22 mm ID x 30 cm L; Sample: protein standards;
Elution: 0.06 mol/L phosphate buffer, pH 7, in 0.06 mol/L KCl;
Legend: Ve=elution volume, Vc=column volume
figure 5
Calibration curves for globular proteins on TOYOPEARL HW-type resins
Colu n:
Sample:
Elution:
Legend:
22mm ID x 30cm
protein standards
0.06mol/L phosphate buffer, pH 7, in 0.06mol/L KCl
V
e
=elution volume, V
c
=column volume
Calibration curves for globular proteins on Toyopearl HW-type resins
V
e
/ V
c
HW-75
HW-65
HW-55
HW-50
HW-40
MW (Da)
10
7
10
6
10
5
10
4
10
3
10
2
10
0.30 0.40 0.50 0.60 0.70 0.80
Properties and molecular weight separation ranges for TOYOPEARL HW-type resins
(HW = Hydrophilic, water-compatible polymeric base resins)
Molecular weight of sample (Da)
TOYOPEARL
Particle
Pore
PEG and PEO
Dextrans
Globular
resin
size (µm)
size (nm)
proteins
HW-40S
20 - 40
5
1 x 10
2
- 3 x 10
3
1 x 10
2
- 7 x 10
3
1 x 10
2
- 1 x 10
4
HW-40F
30 - 60
5
HW-40C
50 - 100
5
HW-50S
20 - 40
12.5
1 x 10
2
- 1.8 x 10
4
5 X 10
2
- 2 x 10
4
5 x 10
2
- 8 x 10
4
HW-50F
30 - 60
12.5
HW-55S
20 - 40
50
1 x 10
2
- 1.5 x 10
5
1 x 10
3
- 2 x 10
5
1 x 10
3
- 7 x 10
5
HW-55F
30 - 60
50
HW-65S
20 - 40
100
5 x 10
2
- 1 x 10
6
1 x 10
4
- 1 x 10
6
4 x 10
4
- 5 x 10
6
HW-65F
30 - 60
100
HW-75F
30 - 60
>100
4 x 10
3
- 5 X 10
6
1 x 10
5
- 1 x 10
7
5 x 10
5
- 5 x 10
7
TABLe II
