sec
SEC
9
SUMMARY OF TSKgel SIZE EXCLUSION COLUMN LINES
Column line
TSKgel SW / SW
XL
/
SuperSW / UltraSW
TSKgel PW / PW
XL
TSKgel Alpha /
TSKgel SuperAW
TSKgel H
Particle composition
Silica
Polymethacrylate
highly crosslinked Poly-
methacrylate
PS-DVB
No. of available pore
sizes
3/2/1
7
5
6
pH stability
2.5 - 7.5
2.0 - 12.0
2.0 - 12.0
1.0 - 14.0
Solvent compatibility
100% polar
50% polar
100% polar and
nonpolar
100% nonpolar, limited
polar
Max. temperature
30°C
80°C*
80°C
60-80°C (H
XL,
SuperHZ)
140°C (H
HR
and SuperH)
220°C HHR HT2
Pressure** (MPa)
1.0-12.0
1.0 - 4.0
2.0 - 4.0
1.5-6.0
Application focus
proteins
water soluble polymers
intermediate polar
polymers
organic-soluble
polymers
*
Except for the TSKgel G-DNA-PW, which can be operated up to 50°C and the 55 mm ID TSKgel PW-type columns, which can be
operated up to 60°C. When operating below 10°C, reduce the flow rate to ensure that the maximum pressure is not exceeded.
** Depends on column dimensions and particle size.
Note: The operating conditions and specifications for each column are listed on the Operating Conditions and Specifications sheet (OCS)
shipped with the column and in the Ordering Information section at the end of each section.
GEL PERMEATION CHROMATOGRAPHY (GPC)
GPC plays an important role in the characterization of polar organic-
soluble and organic-soluble polymers in consumer, chemical, and
petrochemical industries. GPC is often used to determine the relative
molar mass of polymer samples as well as the distribution of molar
masses.
Application area:
Water- and organic-soluble polymers
Base material: highly crosslinked polymethacrylate
• Alpha
• SuperAW
TSKgel Alpha and SuperAW columns are compatible with a wide
range of solvents and were developed for the GPC analysis of
polymers of intermediate polarity, soluble in water, buffers and
many organic solvents. TSKgel SuperAW columns are based on the
same chemistry as TSKgel Alpha columns but have smaller particle
sizes and shorter, narrower column dimensions for high throughput
applications.
For the GPC analysis of organic-soluble polymers, Tosoh developed
TSKgel H series, filled with polystyrene/divinylbenzene polymer
particles. Each line of columns within the TSKgel H series differs in
degree of inertness and operating temperature range. The proprietary
multi-pore particle technology applied in some linear GPC columns
ensures a wide pore size distribution in each particle leading to
calibration curves with excellent linearity.
Application area:
Organic-soluble polymers
Base material: polystyrene
Ultra-low adsorption columns with limited solvent range
• SuperHZ (high throughput)
• SuperMultiporeHZ
• H
XL
(conventional)
Low adsorption columns with expanded solvent range
• SuperH (high throughput)
• H
HR
(conventional)
High temperature GPC columns
• GMH
HR
HT/HT2
