Lab Catalog - page 14

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10
Column Selection Guide for TSKgel Gel Filtration Columns
SAMPLE COLUMN SELECTION SELECTION CRITERIA
FIRST CHOICE ALTERNATIVE
Carbohydrates polysaccharides TSKgel GMPW XL
TSKgel SuperMultiporePW
TSKgel G5000PWXL &
TSKgel G3000PWXL
large pore size, small particles, linear
calibration curve, high resolving
power
oligosaccharides TSKgel G-Oligo-PW
TSKgel SuperOligoPW
TSKgel G2500PWXL small particles, high resolving
power
Nucleic
acids
DNA fragments large TSKgel G-DNA-PW or
TSKgel G5000PW XL
large pore size, small particles,
high resolving power
medium
and small
TSKgel G4000SW XL,
TSKgel BioAssist G4SWXL
TSKgel SuperSW3000 or
TSKgel G3000SW XL TSKgel BioAssist G3SWXL
suitable pore sizes
RNA TSKgel G4000SW XL
TSKgel SuperSW3000 or
TSKgel G3000SW XL
TSKgel BioAssist G4SWXL
TSKgel BioAssist G3SWXL
suitable pore sizes
oligonucleotides TSKgel G2500PW XL small pore size, ionic interaction
Proteins small to medium
sized proteins
TSKgel SuperSW3000
TSKgel G3000SW XL
TSKgel BioAssist G3SWXL
TSKgel G4000SW XL
TSKgel SuperSW2000 or
TSKgel G2000SW XL
TSKgel G3000PW XL /
G4000PWXL
TSKgel BioAssist G4SWXL
TSKgel BioAssist G2SWXL
small particles
small to medium range pore sizes
antibodies TSKgel SuperSW mAB HR/HTP
TSKgel UltraSW Aggregate
fragments/monomer & dimer
higher aggregates
large proteins low density
lipoprotein
TSKgel G6000PW XL or
TSKgel G5000PW XL
large pore sizes
gelatin TSKgel GMPW XL
TSKgel SuperMultiporePW-M
TSKgel G3000SW XL
TSKgel G5000PWXL &
G3000PWXL
large pore size,
linear calibration curve
Peptides large TSKgel SuperSW3000
TSKgel G3000SW XL
TSKgel BioAssist G3SWXL or
TSKgel G2000SW XL
TSKgel SuperSW2000 /
TSKgel G3000PWXL
TSKgel BioAssist G2SWXL
small to medium
range pore size, versatile
small TSKgel G2500PW XL TSKgel SuperSW2000 /
TSKgel G2000SWXL
linear calibration curve,
high resolving power
Viruses TSKgel G6000PW XL or
TSKgel G5000PW XL
TSKgel SuperMultiporePW-H
large pore size, high resolving
power
Synthetic
polymers
TSKgel GMPW XL or
TSKgel Alpha-M
TSKgel SuperMultiporePW
TSKgel G5000PWXL &
G3000PWXL / TSKgel Alpha-
5000 & Alpha-3000
large pore size, low adsorption,
linear calibration curve
cationic TSKgel G3000PW XL-CP
TSKgel G5000PW XL-CP
TSKgel G6000PW XL-CP
medium to large pore size, low
adsorption, linear calibration
curve
Synthetic
oligomers
nonionic TSKgel G-Oligo-PW
TSKgel G2500PW XL or
TSKgel Alpha-2500
TSKgel SuperOligoPW and
TSKgel SuperMultiporePW-N
TSKgel G2500PW /
TSKgel SuperAW2500
small pore size, high resolving
power
anionic TSKgel G2500PW XL or
TSKgel Alpha-2500
TSKgel G2500PW /
TSKgel SuperAW2500
small pore size, ionic interaction