tosoh bioscience
chromatography catalog
3
tosoh bioscience
chromatography
catalog
TSKgel columns
TSKgel columns are known worldwide for their reliability and suitabil-
ity for a variety of chromatographic applications. Applications using
TSKgel columns are continuously published in the scientific journals
and are listed in the current U.S. Pharmacopoeia (see Appendix C).
Prepacked columns contain TSKgel media designed for the analysis of
proteins, peptides, biopolymers and lowmolecular weight compounds
by size exclusion, ion exchange, hydrophobic interaction, reversed
phase, affinity and normal phase chromatography. The packings in
the columns are either silica-based or polymeric-based material, in
particle sizes ranging from 2 µm to 20 µm.
Columns are available in analytical to preparative sizes, in stainless
steel, PEEK®, or glass. To ensure specified column performance and
to maximize the longevity of your Tosoh columns, please note the
guidelines found in Appendix A for the use, cleaning, rehydration, and
storage of your TSKgel columns.
ToyoScreen Process Development columns
ToyoScreen Process Development columns are available as an addi-
tional tool for convenient scale-up. These are easy-to-use, pre-packed
columns containing Tosoh Bioscience‘s most popular TOYOPEARL
resins. They provide a convenient, low-cost product for the evaluation
of TOYOPEARL ligand chemistries. The most popular resins are also
available in RoboColumn (
®
) format for high throughput screening on
robotic workstations and as 5 mL (8 mm ID x 10 cm) ready-to-use Mini-
Chrom columns. Both formats are packed by Atoll GmbH, Weingarten.
TOYOPEARL and TSKgel LabPak Media
LabPak products are small package sizes of TOYOPEARL and TSK-
gel bulk media products. Typically they contain three or four differ-
ent ligand types offered for a particular chromatography mode. They
are useful for developmental scientists who wish to familiarize them-
selves with resin physical properties in differing buffer systems. The
larger resin amounts in LabPak products allow the packing of wider
bore and longer columns than what is available in the ToyoScreen
products. This helps the developmental scientist or engineer to mea-
sure more accurately, under actual packing conditions, the resin‘s
dynamic binding capacity, and selectivity.
TSKgel resins
The same media used in TSKgel columns are also available in bulk.
They are offered in particle sizes of 20 µm and 30 µm, for ion exchange
and hydrophobic interaction chromatography. TSKgel media are the
most efficient packing materials available from Tosoh Bioscience for
process chromatography. In tandem with their high efficiency, high
mechanical stability and permeability, TSKgel resins are an excellent
choice under medium to high pressure conditions.
TOYOPEARL resins
TOYOPEARL resins are hydrophilic, macroporous, bulk bioprocessing
media suitable for large-scale chromatographic applications. Be-
cause of their polymeric backbone structure, the rigid TOYOPEARL
packings assure excellent pressure/flow characteristics. The media
are stable over the pH range of 2-12 for normal operating conditions
and pH 2-14 for cleaning conditions. The particle sizes are: 20-50 µm
superfine grade for the highest performance, 40-90 µm medium grade
for economical purification, and 50-150 µm coarse grade for capture
chromatography. Large pore sizes ensure greater capacity for high
molecular weight molecules, while allowing faster separation and re-
cycling times. TOYOPEARL media are available for size exclusion, ion
exchange, mixed-mode, hydrophobic interaction, and affinity chroma-
tography.
For predictable results in scale-up, TOYOPEARL resins are based on
the same chemistry as the prepacked TSKgel columns. This allows
the seamless scale-up of methods developed on TSKgel columns to
TOYOPEARL, without additional optimization at pilot scale. In addi-
tion, TOYOPEARL resins are also available in the ToyoScreen Process
Development columns for convenient scouting and methods develop-
ment.
