39
MX
HIGH BINDING CAPACITY AT HIGH CONDUCTIVITIES
TOYOPEARL MX-Trp-650M exhibits dynamic binding
capacities (DBC) for immunoglobulin G as high as 90-100
g/L at standard flow rates (Figure 3). At elevated flow rates/
shorter residence times the binding capacity still remains
high. Table 1 shows the DBC of the new resin at two feed-
stock conductivities: 12 mS/cm and 17 mS/cm. For compar-
ison purposes, data for another agarose based multimodal
cation exchanger (Brand M) is also shown. For both conduc-
tivity levels the new TOYOPEARL MX-Trp-650M resin shows
much higher DBCs than the agarose based resin.
Mechanical stability
The rigid polymer matrix allows high velocities. This
can considerably increase throughput when processing
large volumes of feedstock in process scale operations.
TOYOPEARL MX-Trp-650M is based on the well proven rigid
polymethacrylate matrix used for all TOYOPEARL media.
This matrix exhibits high mechanical stability and creates
less than half the backpressure of agarose based media of
the same particle size (Figure 4).
TOYOPEARL MX-Trp-650M pressure / flow curve
Column size: 22 mm ID x 20 cm; Eluent: distilled water
TOYOPEARL MX-Trp-650M
Agarose based multimodal
cation exchanger
IgG (g/L)
60
0
20
40
80
100
120
12
10
8
6
4
2
0
% breakthrough
212 cm/h
425 cm/h
637 cm/h
849 cm/h
Column: TOYOPEARL MX-Trp-650M (6 mm ID x 4 cm);
Sample: polyclonal human IgG (1 mg/mL) in 0.05 mol/L NaAc + 0.1 mol/L
sodium chloride (pH 4.7); Linear velocity: 212, 425, 637, 849 cm/h; Detec-
tion: UV @ 280 nm
TOYOPEARL MX-Trp-650M DYNAMIC BINDING CAPACITY
figure 3
figure 4
Resin
Particle
size (µm)
DBC
(g/L)
Recovery
%
TOYOPEARL MX-Trp-650M
(12 mS/cm)
75
95
97
TOYOPEARL MX-Trp-650M
(17 mS/cm)
75
48
96
Brand M Agarose
(12 mS/cm)
75
14
86
Brand M Agarose
(17 mS/cm)
75
11
85
Resins: TOYOPEARL MX-Trp-650M, Brand M
Column size: 6 mm ID x 4 cm; Mobile phase: Buffer (12 mS/cm):
0.05 mol/L acetate (pH 4.3, 4.7, 5.0) + 0.10 mol/L NaCl, Buffer
(17 mS/cm): 0.05 mol/L acetate (pH 4.3, 4.7, 5.0) + 0.15 mol/L
NaCl; Flow rate: 1.0 mL/min (212 cm /h); Detection: UV @ 280 nm;
Sample: polyclonal human IgG (1 g/L);
Dynamic binding capacity (DBC) calculated at 10 % breakthrough.
HIGH salt tolerance
table III
mixed-mode
chromatography
