47
HIC
Particle size optimization
TOYOPEARL and TSKgel PW-type methacrylic base beads
incorporate the same polymer chemistry (see Figure 6 or
refer to SEC section for a more detailed discussion) and are
available in a variety of particle sizes:
100 µm
TOYOPEARL C-grade
Capture
65 µm
TOYOPEARL M-grade Intermediate
Purification
35 µm TOYOPEARL S-grade
Intermediate
Purification/
Polishing
30 & 20 µm TSKgel PW-type
High Resolution
In many cases analytical columns are available packed
with 10 micron particles having the same selectivity as the
process media. Figure 7 shows the variety of ligands and
particle sizes available for HIC process-scale applications.
This figure also depicts where each particle size is used in
a chromatographic manufacturing train (i.e. for capture,
intermediate purification, or polishing).
Mechanical stability
The semi-rigid polymeric backbone of TOYOPEARL and
TSKgel HIC resins permits high flow rates for maximum
throughput and productivity. TOYOPEARL resins may
be operated up to 3 bar and TSKgel PW-type resins may
be operated up to 20 bar. The pressure-flow characteris-
tics for each particle size grade of TOYOPEARL Phenyl-650
resins are shown in Figure 8. The beads are compatible with
both fixed bed and dynamic axial compression columns.
Chemical stability
The polymeric structure of these products also makes
them resistant to a wide range of pH conditions and ionic
strengths. In addition, the hydroxylated surface of the base
bead reduces non-specific binding of proteins.
hydrophobic interaction
chromatography
Capture
Intermediate
Purification
Polishing
QC
Same selectivity HPLC columns
are available for most process media
Process step
Bead size
Process media
100 µm
TOYOPEARL SuperButyl-650C
TOYOPEARL Hexyl-650C
TOYOPEARL Butyl-650C
TOYOPEARL Phenyl-650C
65 µm
TOYOPEARL Butyl-600M
TOYOPEARL Phenyl-600M
TOYOPEARL PPG-600M
TOYOPEARL Butyl-650M
TOYOPEARL Phenyl-650M
TOYOPEARL Ether-650M
35 µm
30 µm
TOYOPEARL Butyl-650S
TOYOPEARL Phenyl-650S
TOYOPEARL Ether-650S
TSKgel Phenyl-5PW (30)
TSKgel Ether-5PW (30)
TSKgel Phenyl-5PW (20)
TSKgel Ether-5PW (20)
TSKgel Phenyl-5PW 2 mm ID x 7.5 cm L
TSKgel Ether-5PW 2 mm ID x 7.5 cm L
20 µm
10 µm
Pure Product
Feedstock
Figure 7
