TSKgel G-DNA-PW
TSKgel G-DNA-PW is a specialty column designed for the separation of large polynucleotides such as DNA and RNA fragments of 500-5000 base pairs. TSKgel G-DNA-PW is a smaller particle size version of TSKgel G6000PWXL.
The TSKgel G-DNA-PW column has a base material of hydroxylated polymethacrylate, can be used in a maximum of 20% organic, and is shipped in water.
Double stranded DNA calibration curves for TSKgel G-DNA-PW column |
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Columns: TSKgel G-DNA-PW, 10 µm, 7.8 mm ID × 30 cm × 4
Mobile phase: 0.2 mol/L H2O with 0.3 mol/L NaCl in 0.1 mol/LTris-HCl,
pH 7.5, + 1 mmol/L EDTA
Flow rate: 0.15 mL/min
Detection: UV @ 260 nm
Sample: Eco RI and Bst NI-cleaved pBR322 DNA, void volume
determined with λ-DNA |
Analysis of large DNA fragments |
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Columns: TSKgel G-DNA-PW, 10 µm, 7.8 mm ID × 30 cm × 4
Mobile phase: H2O with 0.3 mol/L NaCl in 0.1 mol/LTris-HCl,
pH 7.5, + 1 mmol/L EDTA
Flow rate: A: 0.15 mL/min, B: 0.5 mL/min
Detection: UV @ 260 nm
Sample: 60 µL of Eco RI and Bst NI - cleaved pBR322 DNA, base pairs:
1. 4362
2. 1857
3. 1060 & 928
4. 383
5. 121
6. 13 |