TSKgel SuperSW2000
Featuring 4 μm particles, the TSKgel SuperSW2000 column provides high efficiency for peptides and proteins. The 12.5 nm pore size results in a fractionation range up to 150,000 Da for globular proteins. The narrow 4.6 mm inner diameter of the TSKgel SuperSW2000 column provides higher sensitivity in sample-limited cases and to cut down on solvent use.
It is important to employ an HPLC system that is optimized with regards to extra-column band broadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns.
Analysis of Peptides |
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Column: TSKgel SuperSW2000, 4 µm, 4.6 mm ID × 30 cm
Mobile phase: 0.1% TFA in 45% aq. ACN
Flow rate: 0.35 mL/min
Detection: UV @ 210 nm
Injection vol: 3 µL
Samples: Peptide P12: Tyr-Gly-Gly-Phe-Met-Arg-Gly-Leu
Peptide P16: Trp-Gly-Gly-Tyr
Peptide P19: Gly-Trp-Gly
Peptide P20: H-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-
Gly-Lys-Gly-Lys-Arg-Arg-Pro-Val-Lys-Val-Tyr-Pro-OH
Peptide P22: H-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-
Cys-OH
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