TSKgel G4000SW
As the first generation of silica-based GFC columns, the TSKgel G4000SW columns provide excellent separation of proteins and other biomolecules.
TSKgel G4000SW columns are available in 13 μm particle size in glass and stainless steel analytical columns and 17 μm, 21.5 mm ID x 30 cm and 60 cm columns for semi-preparative purifications.
Separation of total E. coli RNA
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Columns: TSKgel G4000SW, 13 µm, 7.5 mm ID × 30 cm × 2
TSKgel G4000SW, 17µm, 7.5 mm ID × 30 cm × 2
Mobile phase: 0.13 mol/L NaCl in 0.1 mol/L phosphate buffer, pH 7.0, + 1 mmol/L EDTA
Flow rate: 1.0 mL/min
Detection: UV @ 260 nm
Injection vol.: 5 µg
Sample: 0.1 mL of 1:10 diluted solution of total E. coli RNA:
1. 23s rRNA (1.1 × 10^6 Da)
2. 16s rRNA (5.6 × 10^5 Da)
3. 5s rRNA (3.9 × 10^4 Da)
4. 4s rRNA (2.5 × 10^4 Da) |