TSKgel SuperSW mAb HTP
The TSKgel SuperSW mAb HTP gel filtration column is designed with the same pore-controlled technology as the TSKgel SuperSW mAb HR column and is specialized for fast run times. HTP stands for “high throughput,” and the TSKgel SuperSW mAb HTP column's reduced internal diameter and length (4.6 mm ID x 15 cm) allows for high resolution between the mAb dimer and monomer in less time. The resolution is equivalent to that of the TSKgel G3000SWXL column, but in half of the analysis time.
The TSKgel SuperSW mAb HTP column is compatible with both HPLC (optimized with regard to dead volume) and UHPLC systems because of its ability to deliver low pressure drops even at high flow rates.
High speed separation of therapeutic mAb
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Column: TSKgel SuperSW mAb HTP, 4 µm, 4.6 mm ID × 15 cm
Mobile phase: 0.2 mol/L phosphate buffer, pH 6.7 + 0.05% NaN3
Flow rate: 0.50 mL/min, 0.35 mL/min
Detection: UV @ 280 nm
Temperature: 25 ˚C
Sample: monoclonal antibody-2 (mouse-human chimeric IgG, Erbitux®), 5 µL |
Analysis of IgG monomer, dimer and fragments
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Columns: A. TSKgel SuperSW mAb HTP, 4 µm, 4.6 mm ID × 15 cm
B. TSKgel SuperSW mAb HR, 4 µm, 7.8 mm ID × 30 cm
Mobile phase: 200 mmol/L phosphate buffer + 0.05% NaN3, pH 6.7
Flow rate: A: 0.35 mL/min; B: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: 25 ºC
Injection vol.: A: 5 μL; B: 10 μL
Sample: 10 g/L IgG digested with papain for 0-24 hr
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