TSKgel G2000SWXL
Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel G2000SWXL, 5 μm columns are an excellent choice for small proteins and peptide separations. The fractionation range is applicable for globular protein samples up to 150,000 Da. These columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.
Also available within the TSKgel G2000SWXL column line is a BioAssist column made of PEEK housing material to reduce sample adsorption to stainless steel or glass, and QC-PAK columns, which are 15 cm in length with 5 µm particles and offer the same resolution in half the time as the 30 cm TSKgel G2000SWXL column.
Separation of crude extract of rat liver (10 µL)
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Column: TSKgel G2000SWXL, 5 µm, 7.8 mm ID × 30 cm
Mobile phase: 0.05 mol/L phosphate buffer, pH 7.0 + 0.3 mol/L NaCl
Flow rate: 1 mL/min
Detection: UV @ 220 nm
Temperature: 25 °C
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