TSKgel UP-SW Columns

TSKgel UP-SW Columns

The TSKgel UP-SW product line consists of three columns differing in pore size, which allows for the analysis of small to very large molecular weight biopharma products. Available in 4.6 mm ID x 15 and 30 cm lengths, TSKgel UP-SW columns are compatible with both UHPLC and HPLC systems and require less sample while delivering higher sensitivity compared to 7.8 mm ID TSKgel SEC columns.

As shown in the figure, the calibration curves show differing slopes that create distinct separation ranges for each of the columns within the TSKgel UP-SW product line. The calibration curve for the TSKgel UP-SW2000 column is similar to that of the TSKgel G2000SWXL column curve, implying a better resolution of small proteins, peptides and oligonucleotides. The TSKgel UP-SW3000 column shows a shallower slope around the molecular weight region of γ-globulin, making this column ideal for the analysis of monoclonal antibodies.  The UP-SW Aggregate column demonstrates the shallowest slope of the calibration curve of proteins up to the high molecular weight region.

Calibration curves of standard proteins

Column:           A. TSKgel UP-SW2000, 2 µm, 4.6 mm ID × 30 cm                          B. TSKgel UP-SW3000, 2 µm, 4.6 mm ID × 30 cm                          C. TSKgel UP-SW Aggregate, 3 µm, 4.6 mm ID × 30 cm Mobile phase: 100 mmol/L sodium phosphate buffer, pH 6.7, + 100 mmol/L sodium sulfate + 0.05 % sodium azide Flow rate:        0.35 mL/min Detection:       UV @ 280 nm Temperature:  25 °C Injection vol.: 10 μL Samples:         1. thyroglobulin (MW 640,000) (1'. thyroglobulin dimer)                          2. γ - globulin (MW 155,000) (2'. γ - globulin dimer)                          3. ovalbumin (MW 47,000)                          4. ribonuclease A (MW 13,700)                          5. p - amino benzoic acid (MW 137)