TSKgel SuperHZ2500
TSKgel SuperHZ2500 GPC columns are packed with rigid, porous, 3 μm polymer beads. These ultra-fine particles provide high resolution for the non-aqueous separation of solvent-soluble oligomers with molar masses of less than 20,000. The maximum operating temperature for the TSKgel SuperHZ2500 columns are 60 °C. The THF shipping solvent may be replaced by a limited number of organic solvents.
TSKgel SuperHZ2500 columns are ideal for high throughput, high efficiency GPC applications such as those encountered in combinatorial chemistry experiments. These columns feature ultra-low sample adsorption, i.e., the columns show true size exclusion behavior for most polymers.
TSKgel SuperHZ2500 column dimensions are 6 mm ID × 15 cm and 4.6 mm ID × 15 cm versus 7.8 mm ID × 30 cm for conventional GPC columns. The smaller column dimensions translate to a reduction of peak volume by a factor of 3.4 (6 mm ID) and a factor of 5.8 (4.6 mm ID) versus the same component eluting from a corresponding TSKgel G2500HXL column. Thus, your HPLC system may require optimization of components that can give rise to extra-column band broadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.
Comparison of analysis on TSKgel SuperHZ and TSKgel HXL columns
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Columns: A. TSKgel SuperHZ columns (4000, 3000, 2500), 4.6 mm ID × 15 cm × 3
B. TSKgel HXL columns (4000, 3000, 2500), 7.8 mm ID × 30 cm × 3
Mobile phase: THF
Flow rate: A. 0.35 mL/min B. 1.0 mL/min
Detection: RI
Temperature: 40 °C
Injection vol.: A. 5 µL B. 30 µL
Sample: phenolic resin
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