Normal Phase/HILIC Chromatography Columns
TSKgel Amide-80 columns are packed with 2, 3, 5, or 10 µm spherical silica particles that are covalently bonded with carbamoyl groups. TSKgel Amide-80 columns possess superior stability in aqueous/organic solvent systems. In addition, it is ideally suited for sensitive LC/MS analysis of water-soluble polar compounds because the high organic content in the mobile phase provides rapid evaporation of solvent during electrospray ionization. Also available within this line is a TSKgel Amide-80 HR column which offers high specifications for plate count (N = >18,000).
TSKgel NH2-100 amino columns stand out by providing much improved chemical stability, a prerequisite for achieving reproducible and reliable results. These columns are unique in that the bonded phase ligand not only has a terminal primary amino group as expected, but that the spacer also incorporates secondary as well as tertiary amino groups. Anionic compounds are retained on the column by ionic interaction. This allows for the use of gradients in salt concentration in addition to gradient elutions with acetonitrile.