TSKgel CM-STAT
TSKgel CM-STAT columns are packed with 7 and 10 µm non-porous polymer particles of which the surface consists of long hydrophilic chains of open access network of multi-layered carboxymethyl anion exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity, low operating pressure, and rapid analysis.
Applications for the TSKgel CM-STAT columns include the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, and peptide digests.
Antibody Analysis |
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Columns: A: TSKgel CM-STAT, 7 µm, 4.6 mm ID × 10 cm
B: ProPac WCX-10, 10 µm, 4 mm ID × 25 cm
Mobile phase: A: 20 mmol/L MES, pH 6.0
B: 20 mmol/L MES + 0.5 mol/L NaCl, pH 6.0
Gradient: A: 0 min (10%B) 15 min (30%B) 15 min (100%B)
17 min (0%B) 17 min (10%B) 21 min (10%B)
B: 0 min (10%B) 30 min (30%B) 30 min (100%B) 32 min (100%B)
32 min (10%B) 36 min (10%B)
Flow rate: A: 1.0 mL/min B: 2.0 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 20 µL
Samples: monoclonal antibodies (mAb A through E) |
Separation of charge isomers |
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Column: TSKgel CM-STAT, 7 µm, 4.6 mm ID × 10 cm
Mobile phase: A: 50 mmol/L sodium acetate buffer, pH 5.0
B: 30 mmol/L sodium acetate buffer
(pH not adjusted)
Column equilibrated with mobile phase A, the sample is injected, then eluted stepwise to 100% mobile phase B
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 10 µL
Sample: purified mAb
Sample concentration: 1 g/L |