TSKgel SuperH2500
The TSKgel SuperH2500 GPC column is packed with rigid, porous, polymer beads. It is for the non-aqueous separation of oligomers and polymers with molar masses of less than 20,000. The maximum operating temperature for the TSKgel SuperH2500 column is 140 °C. This column is shipped in THF, which can be exchanged for a wide variety of organic solvents.
TSKgel SuperH2500 is a high efficiency/high throughput version of the conventional TSKgel G2500HHR column. Both column types are based on the same bead chemistry. The volume of the 6 mm ID × 15 cm TSKgel SuperH2500 column is 3.4 times smaller than that of a conventional 7.8 mm ID × 30 cm column. As a result, peak volumes will be proportionally smaller on this column compared to a corresponding TSKgel HHR column. Thus, your HPLC system may require optimization of components that can give rise to extra-column band broadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.
Separation of standard polystyrenes using a TSKgel SuperH2500 column
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Column: TSKgel SuperH2500, 3 µm, 6 mm ID × 15 cm
Mobile phase: THF, chloroform, DMF, carbon tetrachloride
Flow rate: 0.6 mL/min
Temperature: 25 °C
Detection: UV/VIS @ 254 nm or 270 nm
Samples: 1) polystyrene (1.9 × 10^5 Da), 2) polystyrene (9.1 x 10^4 Da)
3) polystyrene (2,800 Da), 4) polystyrene A-500
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