TOYOPEARL NH2-750F
TOYOPEARL NH2-750F is a salt tolerant anion exchange resin capable of aggregate removal in both flow-through and binding and elution modes. The resin is composed of polymethacrylate beads that have been functionalized with proprietary primary amine (NH2) strong anion exchange groups.
TOYOPEARL NH2-750F is ideal for process scale applications ranging from the purifications of proteins from biological feedstock (mammalian cell culture, plasma, bacterial feedstock, etc.) without dilution to the intermediate or final purification of monoclonal antibodies (mAbs) where aggregates and other impurities are removed from the target of interest.
TOYOPEARL NH2-750F is available in 45 µm particle size (F-grade) and exhibits typical binding capacities of 70 g/L for BSA. It maintains its high capacity at increased linear velocities and has good pressure-flow characteristics. This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
Structure of TOYOPEARL NH2-750F
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Product attributes
| Pore size (mean): |
> 100 nm |
| Particle size (mean): |
45 µm (F-grade) |
| Pressure rating: |
0.3 Mpa |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-13 |
| Stability data: |
10 years (estimated) |
Comparative salt tolerance of anion exchange resins
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Resins: various
Column size: 5 cm ID × 5 cm
Mobile phase: A: 20 mmol/L Tris-HCl, pH 8.0 B: mobile phase A + 2.0 mol/L NaCl
Gradient: 0 - 100% B (120 min)
Flow rate: 300 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Sample: BSA (1.0 g/L) |
Separation of aggregates from IgG1 monomer on TOYOPEARL NH2-750F
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Resin: TOYOPEARL NH2-750F
Column size: 5 cm ID × 5 cm
Mobile phase: A: 20 mmol/L Tris-HCl, pH 8.0 B: mobile phase A + 1.0 mol/L NaCl
Gradient: 0 - 100% B (60 min)
Flow rate: 300 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Sample: mAb (IgG1) 0.5 g/L |