TOYOPEARL AF-Chelate-650M resin carries a chelating ligand, iminodiacetic acid (IDA), that can form stable chelate complexes with selected metal ions such as Cu2+, Ni2+, Zn2+ and Co2+. The resultant resin can be used for immobilized metal affinity chromatography (IMAC). It binds to histidine rich/histidine tagged proteins and to cysteine containing proteins. This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.

Structure of TOYOPEARL AF-Chelate-650M



Product attributes

Pore size (mean): 100 nm
Particle size (mean): 65 µm
Pressure rating: 0.3 Mpa
Shipping buffer: 20% ethanol
pH stability: 2-12
Stability data: 10 years (estimated)

Ordering Information

P/N Description Dimension
0014475 Toyopearl AF-Chelate-650M 25 mL
0019800 Toyopearl AF-Chelate-650M 100 mL
0014907 Toyopearl AF-Chelate-650M 1 L
0014908 Toyopearl AF-Chelate-650M 5 L
0045202 SkillPak 1 AF-Chelate-650M 1 mL column 7 mm ID x 2.5 cm L
0045259 SkillPak 5 AF-Chelate-650M 5 mL column 8 mm ID x 10 cm L
0045342 SkillPak 50 AF-Chelate-650M 50 mL column 2.5 cm ID x 10 cm L
0045343 SkillPak 200 AF-Chelate-650M 200 mL column 5 cm ID x 10 cm L