TOYOPEARL Butyl-600M
TOYOPEARL Butyl-600M is based on a 75 nm (750 Å) pore size polymethacrylate base material bonded with C4 groups. Its pore size has been optimized for the purification of monoclonal antibodies and is between that of TOYOPEARLl Butyl-650 and TOYOPEARL SuperButyl-550 media. This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
TOYOPEARL Butyl-600M is typically used for intermediate purification or polishing steps.
Structure of TOYOPEARL Butyl-600 resins
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Product attributes
| Pore size (mean): |
75 nm |
| Particle size (mean): |
65 µm (M-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
>1-13 |
| Stability data: |
10 years (estimated) |
Comparison of TOYOPEARL -600M resins
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Resins: A. TOYOPEARL PPG-600M
B. TOYOPEARL Phenyl-600M
C. TOYOPEARL Butyl-600M
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.8 mol/L (NH4)2SO4 + 0.1 mol/L sodium phosphate, pH 7.0
Buffer B: 0.1 mol/L sodium phosphate, pH 7.0
Gradient: 60 min linear gradient from buffer A to B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Samples: 1 g/L of: 1. ribonuclease A, 2.lysozyme, 3. α-chymotrypsinogen
Load volume: 100 µL |
Breakthrough curves of polyclonal IgG on various HIC resins
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Resins: A. TOYOPEARL PPG-600M
B. TOYOPEARL Phenyl-650M
C. TOYOPEARL Butyl-600M
D. Competitor Phenyl Agarose
E. TOYOPEARL Phenyl-600M
Column size: 7.8 mm ID × 20 cm
Mobile phase: 0.1 mol/L sodium phosphate, pH 7.0 + 0.8 mol/L (NH4)2SO4
Flow rate: 300 cm/hr (2.4 mL/min)
Detection: UV @ 280 nm
Temperature: 25 °C
Samples: 1.0 g/L polyclonal IgG
DBC was calculated at 10% breakthrough |