TOYOPEARL Butyl-650
TOYOPEARL Butyl-650 is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with C4 groups. This resin has the second highest hydrophobicity of the TOYOPEARL HIC resins. Its pore size is the largest of our three butyl resins. Primary applications are the separation of proteins, their isoforms, and aggregate removal. It is typically used in intermediate purification and polishing steps.
TOYOPEARL Butyl-650 is available in three particle sizes, 35 µm (S-grade), 65 µm (M-grade), and 100 µm (C-grade). This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
Structure of TOYOPEARL Butyl-650 resins
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Product attributes
| Pore size (mean): |
100 nm |
| Particle size (mean): |
100 µm (C-grade)
65 µm (M-grade)
35 µm (M-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
>1-13 |
| Stability data: |
10 years (estimated) |
Typical dynamic binding capacities for humanized mAb
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Binding capacitiy (mg/mL - gel)
(10% breakthrough)
Phenyl-650M 35.2
Butyl-650M 42.1
PPG-600M 44.8
Butyl-600M 48.5 |
Selectivity of TOYOPEARL HIC resins
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| Samples: 1. Ribonuclease A, 2. Lysozyme, 3. α- Chymotrypsinogen |