Hydroxyapatite Chromatography
Ca⁺⁺Pure-HA resin has been sintered at high temperatures for increased mechanical and pH stability, allowing it to withstand the rigors of industrial-scale applications. Ca⁺⁺Pure-HA has a demonstrated dynamic binding capacity (DBC), at 5% breakthrough, of 55 mg mAb/mg resin at 5 minute residence time.
| Mechanical Stability |
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| Caustic Stability |
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Resin: Ca⁺⁺Pure-HA
Column size: 1.1 cm ID × 19.3 cm (dry pack)
2.2 cm ID × 20.3 cm (dry pack)
4.4 cm ID × 19.3 cm (dry pack)
2.2 cm ID × 20.6 cm (wet pack)
10.0 cm ID × 21.3 cm (wet pack)
Mobile phase: 5 mmol/L sodium phosphate, pH 7.2
Linear velocity: as noted
Detection: pressure (MPa) |
| Dynamic Binding Capacity - Human IgG |
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| Resin: Ca⁺⁺Pure-HA
Column size: 6.6 cm ID × 3 cm (1 mL)
Mobile phase: 5 mmol/L sodium phosphate, pH 6.8
Flow rate: 5 min residence time: 0.21 mL/min (36 cm/hr)
3.5 min residence time: 0.3 mL/min (53 cm/hr)
2 min residence time: 0.52 mL/min (91 cm/hr)
1 min residence time: 1.0 mL/min (180 cm/hr)
Detection: UV @ 280 nm (10% breakthrough)
Sample: lysozyme, 1.0 g/L |
| Dynamic Binding Capacity - Lysozyme |
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| Resin: Ca⁺⁺Pure-HA
Column size: 6.6 cm ID × 3 cm (1 mL)
Mobile phase: 10 mmol/L sodium phosphate, pH 6.5
Flow rate: 5 min residence time: 0.21 mL/min (36 cm/hr)
2 min residence time: 0.52 mL/min (91 cm/hr)
Detection: UV @ 280 nm (10% breakthrough)
Sample: human IgG, 1.0 g/L | | | | | | 1 min | 2 min | 3.5 min | 5 min | | Lysozyme | 24.9 g/L | 24.9 g/L | 25.0 g/L | 25.1 g/L | | Human IgG | 32.4 g/L | N/A | N/A | 51.6 g/L | |