TOYOPEARL GigaCap DEAE-650M
TOYOPEARL GigaCap DEAE-650M is a high capacity anion exchange resin composed of polymethacrylate beads that have been chemically modified to provide a higher number of anionic binding sites and functionalized with diethyl amino ethyl (DEAE) weak anion exchange groups.
TOYOPEARL GigaCap DEAE-650 can be used for high throughput capture and intermediate purification of blood products and other proteins. It is available in 75 µm particle size (M-grade). It exhibits typical dynamic binding capacities of 165 g/L for BSA and maintains the high capacity even at increased linear velocities. It also has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step. This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
TOYOPEARL GigaCap DEAE-650M
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Product attributes
| Pore size (mean): |
< 100 nm |
| Particle size (mean): |
75 µm (M-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
3-13 |
| Stability data: |
10 years (estimated) |
Selectivity Comparison
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Resins: TOYOPEARL GigaCap DEAE-650M
TOYOPEARL GigaCap Q-650M
Competitor DEAE
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 50 mmol/L Tris-HCl, pH 8.5
Buffer B: buffer A + 1.0 mol/L NaCl, pH 8.5
Gradient: 120 minutes, 0 - 100% B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Injection vol.: 100 µL
Samples: 1. transferrin, 2.9 g/L 2. ovalbumin, 6.5 g/L 3. trypsin inhibitor, 10.0 g/L
Sample load: 1.94 mg total protein
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