TOYOPEARL SP-650 is a strong cation exchange resin for biomolecule purification. It is composed of polymethacrylate beads that have been functionalized with Sulfopropyl (SP) strong cation exchange groups. TOYOPEARL SP-650 has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.

TOYOPEARL SP-650 can be used for high throughput capture, intermediate purification, and polishing process steps. TOYOPEARL SP-650 is available in three particle sizes: 100 µm (C-grade), 65 µm (M-grade), and 35 µm (S-grade) respectively. If greater resolution is needed, the smaller particle TSKgel SP-5PW media may be used. As both the TOYOPEARL SP-650 and TSKgel SP-5PW resins use the same ligand, the selectivity will be unchanged.

TOYOPEARL SP-650 exhibits typical dynamic binding capacities for lysozyme of 50 g/L (S-grade, M-grade) and 45 g/L (C-grade). It maintains capacity at increased linear velocities and has good pressure-flow characteristics.

Structure of TOYOPEARL SP-650   



Product attributes

Pore size (mean): 100 nm
Particle size (mean): 100 µm (C-grade)
  65 µm (M-grade)
  35 µm (S-grade)
Shipping buffer: 20% ethanol
pH stability: 2-13
Stability data: 10 years (estimated)

Selectivity comparisons of cation exchange resins 

Resins:            A. TOYOPEARL GigaCap S-650M B. TOYOPEARL GigaCap S-650S
                         C. TOYOPEARL SP-650S D. Competitor Product
                         E. Competitor Product F. TSKgel SP-5PW (20)
Column size:  7.5 mm ID × 7.5 cm 
Mobile phase: Buffer A: 20 mmol/L phosphate, pH 7.0 
                         Buffer B: buffer A + 1.0 mol/L NaCl
Gradient:         0-100% buffer B (60 min)
Flow rate:       136 cm/hr (1.0 mL/min)
Detection:       UV @ 280 nmInjection vol.: 20 µL
Samples:         ribonuclease A, 9.8 g/L cytochrome C, 3.6 g/L lysozyme, 6.4 g/L


Breakthrough curves of mono-PEGylated lysozyme using TOYOPEARL cation exchange resins 

Resins:            A. TOYOPEARL SP-650M B. TOYOPEARL CM-650M
                         C. TOYOPEARL SP-550C D. TOYOPEARL GigaCap CM-650M
                         E. TOYOPEARL GigaCap S-650M
Column size:   6 mm ID × 40 mm
Mobile phase: Buffer A: 20 mmol/L phosphate buffer, pH 7.0
                         Buffer B: 20 mmol/L phosphate buffer, pH 7.0 + 0.5 mol/L NaCl
Flow rate:        212 cm/hr (1.0 mL/min)
Detection:        UV @ 280 nm
Sample:           mono-PEGylated lysozyme, 1.0 mg/mL (PEG MW= 5 kDa)

Dynamic binding capacities were determined at 10% breakthrough 


Ordering Information

P/N Description Dimension
0007994 TOYOPEARL SP-650C 250 mL
0014700 TOYOPEARL SP-650C 1 L
0007995 TOYOPEARL SP-650C 5 L
0043202 TOYOPEARL SP-650M 100 mL
0007997 TOYOPEARL SP-650M 250 mL
0014699 TOYOPEARL SP-650M 1 L
0007998 TOYOPEARL SP-650M 5 L
0019822 TOYOPEARL SP-650S 25 mL
0008437 TOYOPEARL SP-650S 250 mL
0014698 TOYOPEARL SP-650S 1 L
0008438 TOYOPEARL SP-650S 5 L