TOYOPEARL SP-650
TOYOPEARL SP-650 is a strong cation exchange resin for biomolecule purification. It is composed of polymethacrylate beads that have been functionalized with Sulfopropyl (SP) strong cation exchange groups. TOYOPEARL SP-650 has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.
TOYOPEARL SP-650 can be used for high throughput capture, intermediate purification, and polishing process steps. TOYOPEARL SP-650 is available in three particle sizes: 100 µm (C-grade), 65 µm (M-grade), and 35 µm (S-grade) respectively. If greater resolution is needed, the smaller particle TSKgel SP-5PW media may be used. As both the TOYOPEARL SP-650 and TSKgel SP-5PW resins use the same ligand, the selectivity will be unchanged.
TOYOPEARL SP-650 exhibits typical dynamic binding capacities for lysozyme of 50 g/L (S-grade, M-grade) and 45 g/L (C-grade). It maintains capacity at increased linear velocities and has good pressure-flow characteristics.
Structure of TOYOPEARL SP-650
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Product attributes
| Pore size (mean): |
100 nm |
| Particle size (mean): |
100 µm (C-grade)
65 µm (M-grade)
35 µm (S-grade) |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-13 |
| Stability data: |
10 years (estimated) |
Selectivity comparisons of cation exchange resins
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Resins: A. TOYOPEARL GigaCap S-650M B. TOYOPEARL GigaCap S-650S
C. TOYOPEARL SP-650S D. Competitor Product
E. Competitor Product F. TSKgel SP-5PW (20)
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 20 mmol/L phosphate, pH 7.0
Buffer B: buffer A + 1.0 mol/L NaCl
Gradient: 0-100% buffer B (60 min)
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nmInjection vol.: 20 µL
Samples: ribonuclease A, 9.8 g/L cytochrome C, 3.6 g/L lysozyme, 6.4 g/L
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Breakthrough curves of mono-PEGylated lysozyme using TOYOPEARL cation exchange resins
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Resins: A. TOYOPEARL SP-650M B. TOYOPEARL CM-650M
C. TOYOPEARL SP-550C D. TOYOPEARL GigaCap CM-650M
E. TOYOPEARL GigaCap S-650M
Column size: 6 mm ID × 40 mm
Mobile phase: Buffer A: 20 mmol/L phosphate buffer, pH 7.0
Buffer B: 20 mmol/L phosphate buffer, pH 7.0 + 0.5 mol/L NaCl
Flow rate: 212 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: mono-PEGylated lysozyme, 1.0 mg/mL (PEG MW= 5 kDa)
Dynamic binding capacities were determined at 10% breakthrough |