TOYOPEARL AF-rProtein A-650F

TOYOPEARL AF-rProtein A-650F

TOYOPEARL AF-rProtein A-650F is an alkaline resistant protein A affinity resin developed for the capture and purification of monoclonal antibodies. TOYOPEARL AF-rProtein A-650F utilizes an alkaline resistant recombinant Protein A variant. This non-animal derived ligand is bound to the base bead using multipoint attachment. This contributes to both the resin's alkaline resistance and its low ligand leakages in the range of 5-25 ng/mg IgG. Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at short residence times.

TOYOPEARL AF-rProtein A-650F exhibits typical dynamic binding capacities of ≥30 g/L for human IgG at 2 minutes residence time and maintains this capacity at increased linear velocities. The resin maintains its capacity well after repeated CIP exposure to 0.1mol/L NaOH and has reasonable tolerance to CIP with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A-650F also tolerates exposure to above ambient temperatures. The resin is available in 45 µm F-grade particle size.


Structure of TOYOPEARL AF-rProtein A-650F


Product attributes

Pore size (mean): 100 nm
Particle size (mean): 45 µm (F-grade)
Shipping buffer: 20% ethanol
SBC: > 45 g/L
DBC: > 30 g/L

Comparison of linear velocity and pressure curves

Resins:               A. TOYOPEARL AF-rProtein A -650F, 45 µm, 20 cm ID × 32 cm
                            B. TOYOPEARL AF-rProtein A -650F, 45 µm, 20 cm ID × 18 cm
                            C. TOYOPEARL Butyl-650M, 65 µm, 20 cm ID × 21 cm
Column:             QuikScale®, 20 cm ID
Mobile phase:    H2O
Linear velocity: various
Detection:          pressure (MPa)


Temperature stability

Resin:                       TOYOPEARL AF-rProtein A-650F
Mobile phase:          deionized H2O
Autoclave settings: 120 °C, 1.2 atm, 15 min
Heating time:           30 min

TOYOPEARL AF-rProtein is stable at 35 °C for least 3 years (data not shown)


Ordering Information

P/N Description Dimension
0022803 TOYOPEARL AF-rProtein A-650F 10 mL
0022804 TOYOPEARL AF-rProtein A-650F 25 mL
0022805 TOYOPEARL AF-rProtein A-650F 100 mL
0022806 TOYOPEARL AF-rProtein A-650F 1 L
0022807 TOYOPEARL AF-rProtein A-650F 5 L
0022808 TOYOPEARL AF-rProtein A-650F 50 L
0022836 ProteinA-R28 Standard, 10 mg/L (0.5 mL) Kit

Corresponding ELISA kit is supplied by Cygnus Technologies: