TOYOPEARL Super A
TOYOPEARL Super A resin provides a cutting-edge experience in protein A media, blending top-tier critical performance attributes with exceptional operational flexibility.
An enhanced, Animal Origin Free, recombinant Protein A ligand is bound to the polymethacrylate base bead via multipoint attachment resulting in excellent base stability for up to 100 CIP cycles with 0.5 mol/L NaOH and up to 70 CIP cycles with 1 mol/L NaOH while maintaining 90 % of its initial DBC.
TOYOPEARL Super A resin is available in ~45 µm particle size (F-grade) and exhibits dynamic binding capacities of greater than 70 g/L at 5 minutes residence times and greater than 60 g/L at 3 minutes residence time. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to comparable agarose based resins. It is therefore ideally suited for continuous operation using multiple short columns. This resin is also available in pre-packed SkillPak columns to simplify your process development and scale up.
Highlights
- State-of-the-art operational flexibility: Supports high titers with high dynamic binding capacity (DBC), enhanced alkaline stability for rigorous CIP, and excellent pressure flow behavior to increase process efficiency.
- Superior critical process performance: Features mild elution conditions, concentrated elution volumes, and a significant reduction in process impurities, making the process robust, safe, and compliant.
Technical features of TOYOPEARL Super A resin:
| Matrix | Hydroxylated methacrylic polymer |
| Ligand | Modified, code-optimized C domain,
hexameric ligand with multipoint attachment |
| Average particle size | ~45 µm |
| Average pore size | 100 nm |
| Dynamic Binding Capacity | 72 mg mAb/mL resin @ 5 min RT
60 mg mAb/mL resin @ 3 min RT |
| Operating Linear Flow rate | 10 – 600 cm/hr |
| pH stability (Operational) | 3-12 |
| pH stability (CIP) | 2-14 |
| Chemical stability | Stable to all commonly used aqueous buffers in Protein A Chromatography |
| Bacterial Count | Max 100 CFU/mL |
| Endotoxin Concentration | Max 10 EU/mL |
| Delivery conditions | 72% slurry in 20% ethanol |
DBC of TOYOPEARL Super A |
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| Resin : TOYOPEARL Super A
Column Size : 0.6 cm ID x 4.0 cm L |
Alkaline Stability |
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| DBC in dependence of the number of CIP cycles performed with 0.5 M & 1M NaOH at contact time 15 min for each cycle |