TSKgel Protein A-5PW
The TSKgel Protein A-5PW column has been designed for the rapid separation and robust quantification of a variety of antibodies. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes per injection. The column can be used for more than 2,000 injections without regeneration or cleaning.
Packed with hydroxylated methacrylic polymer beads, the TSKgel Protein A-5PW column is designed with a high degree of crosslinking, which allows high flow rate for chromatography while still maintaining chromatographic efficiency, peak width and resolution. The recombinant protein A ligand is a code-modified hexamer of the C domain. An enhanced rProtein A ligand is bound to the TSKgel 5PW base bead via multipoint attachment resulting in excellent base stability in 0.1 mol/L NaOH.
The wide range loading capacity of the TSKgel Protein A-5PW column can accurately determine the titer of mAb at various stages of cell culture media processing. The low level of protein A leaching makes this column a good candidate for small scale purification of mAbs for initial characterization.
Rapid separation of IgG from impurities |
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| Column: TSKgel Protein A-5PW, 20 µm, 4.6 mm ID × 3.5 cm
Binding buffer: 20 mmol/L sodium phosphate buffer, pH 7.4
Elution buffer: 20 mmol/L sodium phosphate buffer, pH 2.5
Stepwise gradient: 0 – 0.5 min: binding buffer
0.5 – 1.1 min: elution buffer
1.1 – 2.0 min: binding buffer
Flow rate: 2 mL/min
Detection: UV @ 280 nm
Sample: 20 µL of CHO cell culture supernatant spiked with
polyclonal IgG (0.5 mg/mL) |
Wide dynamic range with high sample concentration load |
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