TSKgel Phenyl-5PW
TSKgel Phenyl-5PW is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with phenyl groups. The TSKgel Phenyl-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.
Being the most hydrophobic among the three TSKgel HIC columns, TSKgel Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules. Besides the stainless steel standard column format, glass and PEEK column housings are available for samples that may adsorb to stainless steel.
Purification of myristoylated frequenin
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Column: TSKgel Phenyl-5PW, 10 µm, 5 mm ID × 5 cm, glass
Mobile phase: equilibration in 50 mmol/L HEPES, 100 mmol/L KCI, 1 mmol/L DTT,
1 mmol/L MgCl2, 1 mmol/L CaCl2, pH 7.5 step gradient at 12.5 min
to 50 mmol/L HEPES, 1 mmol/L DTT, 1 mmol/L MgCl2, 2 mmol/L
EGTA, pH 7.5
Flow rate: 1 mL/min
Detection: UV @ 280 nm
Temperature: ambient
Sample: E. coli lysate containing myristoylated frequenin, 100 µL |