SOLUTIONS

TSKgel BioAssist S

TSKgel BioAssist S

Specially designed for the separation of large biomolecules such as antibodies, the large pores (130 nm) of the TSKgel BioAssist S cation exchange columns offer superior capacity and resolution at a low column pressure drop. They are constructed via a polymerization technique that allows an equivalent density of sulfopropyl ionic exchange groups to be incorporated into the particle without reducing pore size.

The standard TSKgel BioAssist S column is 4.6 mm ID x 5 cm, while a 10 mm ID x 10 cm semi-preparative column is available for scale-up. Both columns are made of PEEK to reduce protein adsorption.

Analysis of bromelain 

 bioassists_fig1.png
Columns:        A: TSKgel BioAssist S, 7 µm, 4.6 mm ID × 5 cm
                         B: Competitor S, 5 mm ID × 5 cm
Mobile phase: 20 min (TSKgel) or 30 min (Competitor S) linear gradient of NaCl from 0 to                          0.5 mol/L in 20 mmol/L sodium phosphate buffer, pH 7.0
Flow Rate:      0.8 mL/min for TSKgel; 1.0 mL/min for Competitor S
Detection:       UV @ 280 nm
Temperature: 25 °C
Sample:           crude bromelain (C4882, Sigma), 1 mg in 100 L
 

 

Separation of IgM by cation exchange chromatography 

bioassists_fig2.png 
Column:          TSKgel BioAssist S, 7 µm, 4.6 mm ID × 5 cm
Mobile phase: 20 mmol/L sodium phosphate buffer, pH 6.0
Gradient:         0 mol/L - 0.3 mol/L NaCl (5 min) 0.3 mol/L - 0.5 mol/L NaCl (10 min)
Flow Rate:       1 mL/min
Detection:        UV @ 280 nm
Sample:           500 µL of 9.5 mg/mL IgM in mouse ascites fluid; shaded peaks represent                                albumin and IgM respectively 

 

Analysis of protein standards 

bioassists_fig3.png 
Columns:        A: TSKgel BioAssist S, 7 µm, 4.6 mm ID × 5 cm
                         B: Conventional S type product C, 5.0 mm ID × 5 cm
                         C: Conventional S type product D, 4.6 mm ID × 5 cm
Mobile phase: A: 20 mmol/L sodium phosphate buffer, pH 6.5
                         B: 20 mmol/L sodium phosphate buffer containing 1.0 mol/L NaCl, pH 6.5
Gradient:        32 min (A-B)
Flow Rate:      0.8 mL/min
Detection:       UV @ 280 nm
Temperature: 10 °C
Injection vol.: 20 µL
Samples:         1. myoglobin, 1 g/L
                         2. α-chymotrypsinogen A, 2 g/L
                         3. ribonuclease A, 4 g/L
                         4. cytochrome C, 2 g/L
                         5. lysozyme, 2 g/L 

 

Analysis of peptides 

bioassists_fig4.png 
Column:          TSKgel BioAssist S, 7 µm, 4.6 mm ID × 5 cm
Mobile phase: A: 20 mmol/L sodium acetate buffer, pH 5.0
                         B: 20 mmol/L sodium acetate buffer containing 1.0 mol/L NaCl, pH 5.0
Gradient:        A to B linear gradient (20 min)
Detection:       UV @ 280 nm
Temperature: 25 °C 

 

Ordering Information

P/N Description Dimension
0019686 TSKgel BioAssist S, PEEK 4.6 mm ID x 5 cm L
0021411 TSKgel BioAssist S, PEEK 10 mm ID x 10 cm L