SOLUTIONS

TSKgel CM-STAT

TSKgel CM-STAT

TSKgel CM-STAT columns are packed with 7 and 10 µm non-porous polymer particles of which the surface consists of long hydrophilic chains of open access network of multi-layered carboxymethyl anion exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity, low operating pressure, and rapid analysis.

Applications for the TSKgel CM-STAT columns include the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, and peptide digests.

Antibody Analysis 

cm-stat_fig1.png 
Columns:        A: TSKgel CM-STAT, 7 µm, 4.6 mm ID × 10 cm
                         B: ProPac WCX-10, 10 µm, 4 mm ID × 25 cm
Mobile phase: A: 20 mmol/L MES, pH 6.0
                         B: 20 mmol/L MES + 0.5 mol/L NaCl, pH 6.0
Gradient:        A: 0 min (10%B) 15 min (30%B) 15 min (100%B)
                         17 min (0%B) 17 min (10%B) 21 min (10%B)
                         B: 0 min (10%B) 30 min (30%B) 30 min (100%B) 32 min (100%B)
                         32 min (10%B) 36 min (10%B)
Flow rate:       A: 1.0 mL/min B: 2.0 mL/min
Detection:       UV @ 280 nm
Temperature: 25 °C
Injection vol.: 20 µL
Samples:         monoclonal antibodies (mAb A through E) 

 

Separation of charge isomers 

cm-stat_fig2.png 
Column:                         TSKgel CM-STAT, 7 µm, 4.6 mm ID × 10 cm
Mobile phase:                A: 50 mmol/L sodium acetate buffer, pH 5.0
                                        B: 30 mmol/L sodium acetate buffer
                                        (pH not adjusted)
                                        Column equilibrated with mobile phase A, the sample is injected,                                             then eluted stepwise to 100% mobile phase B
Flow rate:                      1.0 mL/min
Detection:                      UV @ 280 nm
Temperature:                25 °C
Injection vol.:                10 µL
Sample:                          purified mAb
Sample concentration: 1 g/L 

 

Ordering Information

P/N Description Dimension
0021965 TSKgel CM-STAT 3 mm ID x 3.5 cm L
0021966 TSKgel CM-STAT 4.6 mm ID x 10 cm L