TSKgel DEAE-NPR
The TSKgel DEAE-NPR column is packed with 2.5 µm, nonporous, hydrophilic polymer beads which are surface modified with a weak anion exchange group. These columns are used for the high speed separation and analysis of proteins and poly- and oligonucleotides. TSKgel DEAE-NPR columns are particularly useful for high resolution separation of DNA digests or fragments.
Analysis of DNA digest |
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Column: TSKgel DEAE-NPR, 2.5 µm, 4. 6 mm ID × 3.5 cm,
with guard column, 4.6 mm ID × 0.5 cm
Mobile phase: A: 0.02 mol/L Tris-HCI, pH 9.0
B: mobile phase A plus 1.0 mol/L NaCl
Gradient: 15 min linear gradient from 48% to 65% mobile phase B
Flow rate: 1.5 mL/min
Detection: UV @ 260 nm
Pressure: 14 MPa
Temperature: 40 ºC
Sample: Hae III digest of pBR322 DNA,
(base pair number for each peak is indicated) |
Detection of HIV-1 PCR-Amplified 130 bp target |
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Column: TSKgel DEAE-NPR, 2.5 µm, 4.6 mm ID × 3.5 cm
Mobile phase: A: 20 mmol/L Tris-HCl with 0.25 mol/L NaCl, pH 7.7
B: 20 mmol/L Tris-HCl with 1 mol/L NaCl, pH 7.7
Flow rate: 1 mL/min
Detection: UV @ 260 nm
Temperature: ambient
Sample: HIV-1 PCR-amplified 130 bp target
Sample load: 20 µL |