TSKgel DNA-STAT
The TSKgel DNA-STAT column is packed with 5 µm, hydrophilic, nonporous polymer particles of which the surface consists of an open access network of multi-layered quaternary ammonium anion exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity, low operating pressure and rapid analysis.
Applications for the TSKgel DNA-STAT columns include the separation of DNA fragments, nucleic acids and nucleotides.
Separation of large DNA fragments
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Column: TSKgel DNA-STAT, 5 µm, 4.6 mm ID × 10 cm
Mobile phase: A: 20 mmol/L Tris-HCl, pH 8.5
B: 0.75 mol/L NaCl in buffer A
Gradient: 0 min (50%B) 25 min (75%B)
Flow rate: 0.8 mL/min
Detection: UV @ 260 nm |