SOLUTIONS

TSKgel SP-5PW

TSKgel SP-5PW

TSKgel SP-5PW columns are packed with porous hydrophilic polymer beads which are surface modified with sulfopropyl strong cation exchange groups. They are used for the separation and analysis of proteins, peptides, and other biologically active molecules.

Selectivity of strong and weak TSKgel cation exchange columns

sp5pw_fig1.png 
Columns:        TSKgel SP-5PW and TSKgel CM-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: 60 min linear gradient from 0 mol/L to 0.5 mol/L NaCl
                         in 0.02 mol/L phosphate, pH 7.0
Flow rate:       1.0 mL/min
Detection:       UV @ 280 nm
Samples:         1. trypsinogen, 2. ribonuclease A
                         3. α-chymotrypsinogen, 4. cytochrome C
                         5. lysozyme 

 

Semi-preparative purification of lipoxidase 

sp5pw_fig2.png 
Column:          TSKgel SP-5PW, 13 µm, 21.5 mm ID × 15 cm
Mobile phase: 120 min linear gradient from 0 mol/L to
                          0.5 mol/L Na2SO4 in 0.02 mol/L acetate, pH 4.5
Flow rate:        4.0 mL/min
Detection:        UV @ 280 nm
Recovery:        lipoxidase activity collected between the two vertical lines was 84%
Sample:           crude lipoxidase, 200 mg 

 

Separation of peptide mixture 

sp5pw_fig3.png 
Column:          TSKgel SP-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: 30 min linear gradient from 0.02 mol/L to 0.5 mol/L
                         phosphate, pH 3.0, in 30% acetonitrile
Flow rate:       1.0 mL/min
Detection:       UV @ 220 nm
Injection vol.: 50 µL
Samples:         2 µg each of:
                        1. oxytocin, 2. met-enkephalin, 3. TRH
                        4. a-MSH, 5. LH-RH (1 µg)
                        6. neurotensin, 7. a-MSH, 8. angiotensin II
                        9. substance P, 10. b-endorphin 

 

Ordering Information

P/N Description Dimension
0018758 TSKgel SP-5PW 2 mm ID x 7.5 cm L
0007161 TSKgel SP-5PW 7.5 mm ID x 7.5 cm L
0007575 TSKgel SP-5PW 21.5 mm ID x 15 cm L
0013062 TSKgel SP-5PW, Glass 5 mm ID x 5 cm L
0008803 TSKgel SP-5PW, Glass 8 mm ID x 7.5 cm L
0014017 TSKgel SP-5PW, Glass 20 mm ID x 15 cm L
0008807 TSKgel SP-5PW Glass Guardgel Kit for P/N 0008803
0016093 TSKgel SP-5PW Prep Guardgel Kit for P/N 0007575
0007211 TSKgel SP-5PW Guardgel Kit for P/N 0007161
0019308 Guard Cartridge Holder for P/N 0042153 2 mm ID x 1 cm L