TSKgel SP-5PW
TSKgel SP-5PW columns are packed with porous hydrophilic polymer beads which are surface modified with sulfopropyl strong cation exchange groups. They are used for the separation and analysis of proteins, peptides, and other biologically active molecules.
Selectivity of strong and weak TSKgel cation exchange columns |
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Columns: TSKgel SP-5PW and TSKgel CM-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: 60 min linear gradient from 0 mol/L to 0.5 mol/L NaCl
in 0.02 mol/L phosphate, pH 7.0
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Samples: 1. trypsinogen, 2. ribonuclease A
3. α-chymotrypsinogen, 4. cytochrome C
5. lysozyme |
Semi-preparative purification of lipoxidase |
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Column: TSKgel SP-5PW, 13 µm, 21.5 mm ID × 15 cm
Mobile phase: 120 min linear gradient from 0 mol/L to
0.5 mol/L Na2SO4 in 0.02 mol/L acetate, pH 4.5
Flow rate: 4.0 mL/min
Detection: UV @ 280 nm
Recovery: lipoxidase activity collected between the two vertical lines was 84%
Sample: crude lipoxidase, 200 mg |
Separation of peptide mixture |
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Column: TSKgel SP-5PW, 10 µm, 7.5 mm ID × 7.5 cm
Mobile phase: 30 min linear gradient from 0.02 mol/L to 0.5 mol/L
phosphate, pH 3.0, in 30% acetonitrile
Flow rate: 1.0 mL/min
Detection: UV @ 220 nm
Injection vol.: 50 µL
Samples: 2 µg each of:
1. oxytocin, 2. met-enkephalin, 3. TRH
4. a-MSH, 5. LH-RH (1 µg)
6. neurotensin, 7. a-MSH, 8. angiotensin II
9. substance P, 10. b-endorphin |