SOLUTIONS

TSKgel SP-NPR

TSKgel SP-NPR

The TSKgel SP-NPR column is packed with 2.5 µm, nonporous hydrophilic polymer beads of which the surface has been modified with a strong cation exchange group. Because this column contains non-porous particles, binding capacity is low compared to porous columns with the same ligand functionality, whereas protein recovery and efficiency is generally very high.

The TSKgel SP-NPR column is used for the separation and analysis of proteins and peptides. This column is particularly useful for adeno-associated viruses and other large biopolymers.

Analysis of purified AAV 

spnpr_fig1.png 
Column:          TSKgel SP-NPR, 2.5 µm, 4.6 mm ID × 3.5 cm
Mobile phase: A. 50 mmol/L HEPES, 1 mmol/L EDTA, 5 mmol/L MgCl, pH 7.5
                         B. 50 mmol/L HEPES, 1 mmol/L EDTA, 5 mmol/L MgCl,
                         pH 7.5 with 0.5 mol/L NaCl; linear gradient from 20% to 100%B
                         in 10 column volumes
Gradient:        0 min (0%B) 2 min (100%B)
Flow rate:       1 mL/min
Detection:       UV @ 280 nm
Sample:           purified adeno-associated virus 

 

pH gradient analysis of hemoglobin A1c 

spnpr_fig2.png 
Column:          TSKgel SP-NPR, 2.5 µm, 4. 6 mm ID × 3.5 cm
Mobile phase: A: 0.02 mol/L MES, and 0.02 mol/L HEPES-NaOH, pH 6.0
                         B: 0.02 mol/L MES, and 0.02 mol/L HEPES- NaOH, pH 8.0
Gradient:        10 min linear gradient from 32% to 75% buffer B
                         (pH 6.66 to pH 7.43)
Flow rate:        1.5 mL/min
Detection:        VIS @ 415 nm
Sample:           hemoglobin standard 

 

Ordering Information

P/N Description Dimension
0013076 TSKgel SP-NPR 4.6 mm ID x 3.5 cm L